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HAMAP rule MF_01576

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General rule information [?]

Accession MF_01576
Dates 22-NOV-2006 (Created)
29-NOV-2023 (Last updated, Version 27)
Name THF_DHG_CYH
Scope(s) Bacteria
Archaea
Template(s) P24186 (FOLD_ECOLI); P51696 (FOLD_PHOPO); Q05213 (FOLD_THEAC); Q9I2U6 (FOLD_PSEAE); Q0PA35 (FOLD_CAMJE); Q5NGF3 (FOLD_FRATT); P9WG81 (FOLD_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_01576 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FOLD
Protein name RecName: Full=Bifunctional protein FolD;
                 Includes:
RecName: Full=Methylenetetrahydrofolate dehydrogenase;
                 EC=1.5.1.5;
                 Includes:
RecName: Full=Methenyltetrahydrofolate cyclohydrolase;
                 EC=3.5.4.9;
Gene name Name=folD;

Comments [?]

FUNCTIONCatalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate.
CATALYTIC ACTIVITY Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)- 5,10-methenyltetrahydrofolate + NADPH; Xref=Rhea:RHEA:22812, ChEBI:CHEBI:15636, ChEBI:CHEBI:57455, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.5;
CATALYTIC ACTIVITY Reaction=(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10- formyltetrahydrofolate + H(+); Xref=Rhea:RHEA:23700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57455, ChEBI:CHEBI:195366; EC=3.5.4.9;
PATHWAYOne-carbon metabolism; tetrahydrofolate interconversion.
SUBUNITHomodimer.
SIMILARITYBelongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Keywords [?]


Gene Ontology [?]

GO:0004477; Molecular function:methenyltetrahydrofolate cyclohydrolase activity
GO:0004488; Molecular function:methylenetetrahydrofolate dehydrogenase (NADP+) activity

Cross-references [?]

PROSITE PS00766; THF_DHG_CYH_1; 1;
PROSITE PS00767; THF_DHG_CYH_2; 1;
Pfam PF00763; THF_DHG_CYH; 1;
Pfam PF02882; THF_DHG_CYH_C; 1;
PRINTS PR00085; THFDHDRGNASE; 1;

Features [?]

From: FOLD_THEAC (Q05213)
Key From To Description Tag Condition FTGroup
BINDING 157 159 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"
[GN]-x-[SG]
BINDING 182 182 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"
[ST]
BINDING 182 182 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"
I
BINDING 223 223 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"
[IVAT]

Additional information [?]

Size range 269-320 amino acids
Related rules None
Fusion Nter: None Cter: None



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