HAMAP rule MF_01619

General rule information [?]

Accession	MF_01619
Dates	16-NOV-2005 (Created) 26-JAN-2023 (Last updated, Version 21)

Name	NAD_malic_enz

Scope

Bacteria; Pseudomonadota

Template

P26616 (MAO1_ECOLI)

Triggered by

HAMAP; MF_01619 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MAO1

Protein name

RecName:		Full=NAD-dependent malic enzyme;
		Short=NAD-ME;
		EC 1.1.1.38;

Gene name

maeA

Comments [?]

Catalytic activity	RHEA:12653: (S)-malate + NAD(+) = CO2 + NADH + pyruvate EC 1.1.1.38
	RHEA:15641: H(+) + oxaloacetate = CO2 + pyruvate EC 1.1.1.38
Cofactor	Mg(2+) Mn(2+) Note: Divalent metal cations. Prefers magnesium or manganese.
Subunit	Homotetramer.
Similarity	Belongs to the malic enzymes family.

Keywords [?]

Metal-binding
NAD
Oxidoreductase

Gene Ontology [?]

GO:0004471; Molecular function: malate dehydrogenase (decarboxylating) (NAD+) activity.
GO:0008948; Molecular function: oxaloacetate decarboxylase activity.

Cross-references [?]

PROSITE	PS00331; MALIC_ENZYMES; 1;
Pfam	PF00390; malic; 1;
	PF03949; Malic_M; 1;
PRINTS	PR00072; MALOXRDTASE; 1;

Features [?]

From: MAO1_ECOLI (P26616)

Key From To Description Tag Condition FTGroup

ACT_SITE 104 104 Proton donor Y

ACT_SITE 175 175 Proton acceptor K

BINDING 246 246 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 E

BINDING 247 247 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 D

BINDING 270 270 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 D

BINDING 157 157 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 R

BINDING 270 270 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 D

BINDING 418 418 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 N

SITE 270 270 Important for activity

Additional information [?]

Size range	528-591 amino acids
Related rules	None
Fusion	None

View rule in raw text format (no links)