HAMAP rule MF_01619
General rule information
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| Accession | MF_01619 |
| Dates | 16-NOV-2005 (Created)
26-JAN-2023 (Last updated, Version 21) |
| Name | NAD_malic_enz |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P26616 (MAO1_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01619 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MAO1 |
| Protein name | RecName: Full=NAD-dependent malic enzyme; Short=NAD-ME; EC=1.1.1.38; |
| Gene name | Name=maeA; |
Comments
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| CATALYTIC ACTIVITY | Reaction=(S)-malate + NAD(+) = CO2 + NADH + pyruvate; Xref=Rhea:RHEA:12653, ChEBI:CHEBI:15361, ChEBI:CHEBI:15589, ChEBI:CHEBI:16526, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.38; |
| CATALYTIC ACTIVITY | Reaction=H(+) + oxaloacetate = CO2 + pyruvate; Xref=Rhea:RHEA:15641, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526; EC=1.1.1.38; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Divalent metal cations. Prefers magnesium or manganese.; |
| SUBUNIT | Homotetramer. |
| SIMILARITY | Belongs to the malic enzymes family. |
Keywords
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Gene Ontology
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| GO:0004471; Molecular function:malate dehydrogenase (decarboxylating) (NAD+) activity |
| GO:0008948; Molecular function:oxaloacetate decarboxylase activity |
Cross-references
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| PROSITE | PS00331; MALIC_ENZYMES; 1; |
| Pfam | PF00390; malic; 1; |
| Pfam | PF03949; Malic_M; 1; |
| PRINTS | PR00072; MALOXRDTASE; 1; |
Features
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| From: MAO1_ECOLI (P26616) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 104 | 104 | /note="Proton donor" | Y | ||||||||
| ACT_SITE | 175 | 175 | /note="Proton acceptor" | K | ||||||||
| BINDING | 246 | 246 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
E | ||||||||
| BINDING | 247 | 247 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
D | ||||||||
| BINDING | 270 | 270 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
D | ||||||||
| BINDING | 157 | 157 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
R | ||||||||
| BINDING | 270 | 270 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
D | ||||||||
| BINDING | 418 | 418 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
N | ||||||||
| SITE | 270 | 270 | /note="Important for activity" | |||||||||
Additional information
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| Size range | 528-591 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |