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Annotation rule MF_01648
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General rule information [?]

Accession MF_01648
Dates 17-APR-2008 (Created)
20-JUL-2020 (Last updated, Version 21)
Name HcaE
Scope
Bacteria; Enterobacterales
Template P0ABR5 (HCAE_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HCAE
Protein name
RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit alpha;
EC 1.14.12.19;
Gene name
hcaE

Comments [?]

Function Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.
Catalytic activity RHEA:20357: 3-phenylpropanoate + H(+) + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD(+)
EC 1.14.12.19
RHEA:25058: (E)-cinnamate + H(+) + NADH + O2 = (2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD(+)
EC 1.14.12.19
Cofactor Fe cation
Note: Binds 1 Fe cation.
Pathway Aromatic compound metabolism; 3-phenylpropanoate degradation.
Subunit This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).
Similarity Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

Keywords [?]


Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0008695; Molecular function: 3-phenylpropionate dioxygenase activity.
GO:0019439; Biological process: aromatic compound catabolic process.

Cross-references [?]

PROSITE PS51296; RIESKE; 1; trigger=PRU00628;
PS00570; RING_HYDROXYL_ALPHA; 1;
Pfam PF00355; Rieske; 1;
PF00848; Ring_hydroxyl_A; 1;
PRINTS PR00090; RNGDIOXGNASE; 1;

Features [?]

From: HCAE_ECOLI (P0ABR5)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     85     85       Iron-sulfur (2Fe-2S)     C  
METAL     87     87       Iron-sulfur (2Fe-2S); via pros nitrogen     H  
METAL     105     105       Iron-sulfur (2Fe-2S)     C  
METAL     108     108       Iron-sulfur (2Fe-2S); via pros nitrogen     H  
METAL     213     213       Iron     H  
METAL     218     218       Iron     H  

Additional information [?]

Size range 453-453 amino acids
Related rules None
Fusion None