HAMAP rule MF_01648

General rule information [?]

Accession	MF_01648
Dates	17-APR-2008 (Created) 19-NOV-2022 (Last updated, Version 24)

Name

HcaE

Scope

Bacteria; Enterobacterales

Template

P0ABR5 (HCAE_ECOLI)

Triggered by

HAMAP; MF_01648 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

HCAE

Protein name

RecName:		Full=3-phenylpropionate/cinnamic acid dioxygenase subunit alpha;
		EC 1.14.12.19;

Gene name

hcaE

Comments [?]

Function	Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.
Catalytic activity	RHEA:20357: 3-phenylpropanoate + H(+) + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD(+) EC 1.14.12.19
	RHEA:25058: (E)-cinnamate + H(+) + NADH + O2 = (2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD(+) EC 1.14.12.19
Cofactor	Fe cation Note: Binds 1 Fe cation.
	[2Fe-2S] cluster Note: Binds 1 [2Fe-2S] cluster per subunit
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation.
Subunit	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).
Similarity	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

Keywords [?]

2Fe-2S
Aromatic hydrocarbons catabolism
Dioxygenase
Iron
Iron-sulfur
Metal-binding
NAD
Oxidoreductase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0008695; Molecular function: 3-phenylpropionate dioxygenase activity.
GO:0019439; Biological process: aromatic compound catabolic process.

Cross-references [?]

PROSITE	PS51296; RIESKE; 1; trigger=PRU00628;
	PS00570; RING_HYDROXYL_ALPHA; 1;
Pfam	PF00355; Rieske; 1;
	PF00848; Ring_hydroxyl_A; 1;
PRINTS	PR00090; RNGDIOXGNASE; 1;

Features [?]

From: HCAE_ECOLI (P0ABR5)

Key From To Description Tag Condition FTGroup

BINDING 85 85 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 C

BINDING 87 87 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 H

BINDING 105 105 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 C

BINDING 108 108 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 H

BINDING 213 213 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H

BINDING 218 218 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H

Additional information [?]

Size range	453-453 amino acids
Related rules	None
Fusion	None

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