HAMAP rule MF_01648
General rule information
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| Accession | MF_01648 |
| Dates | 17-APR-2008 (Created)
12-MAR-2024 (Last updated, Version 25) |
| Name | HcaE |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P0ABR5 (HCAE_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01648 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HCAE |
| Protein name | RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit alpha; EC=1.14.12.19; |
| Gene name | Name=hcaE; |
Comments
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| FUNCTION | Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3- phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid- dihydrodiol (CI-dihydrodiol), respectively. |
| CATALYTIC ACTIVITY | Reaction=3-phenylpropanoate + H(+) + NADH + O2 = 3-(cis-5,6- dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD(+); Xref=Rhea:RHEA:20357, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:51057, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.14.12.19; |
| CATALYTIC ACTIVITY | Reaction=(E)-cinnamate + H(+) + NADH + O2 = (2E)-3-(cis-5,6- dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD(+); Xref=Rhea:RHEA:25058, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15669, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:61451; EC=1.14.12.19; |
| COFACTOR | Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 1 Fe cation.; |
| COFACTOR | Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster per subunit. |
| PATHWAY | Aromatic compound metabolism; 3-phenylpropanoate degradation. |
| SUBUNIT | This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD). |
| SIMILARITY | Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. |
Keywords
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| 2Fe-2S |
| Aromatic hydrocarbons catabolism |
| Dioxygenase |
| Iron |
| Iron-sulfur |
| Metal-binding |
| NAD |
| Oxidoreductase |
Gene Ontology
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| GO:0005506; Molecular function:iron ion binding |
| GO:0008695; Molecular function:3-phenylpropionate dioxygenase activity |
| GO:0019380; Biological process:3-phenylpropionate catabolic process |
Cross-references
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| PROSITE | PS51296; RIESKE; 1; |
| PROSITE | PS00570; RING_HYDROXYL_ALPHA; 1; |
| Pfam | PF00355; Rieske; 1; |
| Pfam | PF00848; Ring_hydroxyl_A; 1; |
| PRINTS | PR00090; RNGDIOXGNASE; 1; |
Features
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| From: HCAE_ECOLI (P0ABR5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 85 | 85 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | ||||||||
| BINDING | 87 | 87 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
H | ||||||||
| BINDING | 105 | 105 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | ||||||||
| BINDING | 108 | 108 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
H | ||||||||
| BINDING | 213 | 213 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
| BINDING | 218 | 218 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
Additional information
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| Size range | 453-453 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |