HAMAP rule MF_01670
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01670 |
| Accession | MF_01670 |
| Dates | 29-SEP-2008 (Created)
3-SEP-2024 (Last updated, Version 18) |
| Name | IolA |
| Scope(s) |
Bacteria Bacillota |
| Template(s) | P42412 (IOLA_BACSU); [ Recover all ] |
| Triggered by |
HAMAP; MF_01670 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | IOLA |
| Protein name | RecName: Full=Malonate-semialdehyde dehydrogenase; Short=MSA dehydrogenase; EC=1.2.1.27; AltName: Full=Methylmalonate semialdehyde dehydrogenase; Short=MMSA dehydrogenase; Short=MSDH; |
| Gene name | Name=iolA; |
Comments
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| FUNCTION | Catalyzes the oxidation of malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively. Is involved in a myo-inositol catabolic pathway. Bicarbonate, and not CO2, is the end-product of the enzymatic reaction. |
| CATALYTIC ACTIVITY | Reaction=3-oxopropanoate + NAD(+) + CoA + H2O = hydrogencarbonate + acetyl-CoA + NADH + H(+); Xref=Rhea:RHEA:76615, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:33190, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.27; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:76616; |
| CATALYTIC ACTIVITY | Reaction=2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+); Xref=Rhea:RHEA:20804, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700, ChEBI:CHEBI:57945; EC=1.2.1.27; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20805; |
| PATHWAY | Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. |
| SUBUNIT | Homotetramer. |
| SIMILARITY | Belongs to the aldehyde dehydrogenase family. IolA subfamily. |
Keywords
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Gene Ontology
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| GO:0004491; Molecular function:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity |
| GO:0018478; Molecular function:malonate-semialdehyde dehydrogenase (acetylating) activity |
| GO:0019310; Biological process:inositol catabolic process |
Cross-references
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| PROSITE | PS00070; ALDEHYDE_DEHYDR_CYS; 1; |
| PROSITE | PS00687; ALDEHYDE_DEHYDR_GLU; 1; |
| Pfam | PF00171; Aldedh; 1; |
| NCBIfam | TIGR01722; MMSDH; 1; |
Features
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| From: IOLA_BACSU (P42412) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 150 | 150 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
A | ||||||||
| BINDING | 152 | 152 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
F | ||||||||
| BINDING | 176 | 176 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
K | ||||||||
| BINDING | 179 | 179 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
E | ||||||||
| BINDING | 180 | 180 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[KR] | ||||||||
| BINDING | 229 | 229 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
T | ||||||||
| BINDING | 251 | 251 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
T | ||||||||
| BINDING | 382 | 382 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
E | ||||||||
| ACT_SITE | 284 | 284 | /note="Nucleophile" | |||||||||
Additional information
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| Size range | 470-520 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |