HAMAP logo

HAMAP rule MF_01679

Send feedback

General rule information [?]

Accession MF_01679
Dates 22-OCT-2008 (Created)
1-JUN-2023 (Last updated, Version 20)
Name Salvage_MtnW
Scope(s) Bacteria
Microcystis
Bacillota
Template(s) O31666 (MTNW_BACSU); Q5L1E2 (MTNW_GEOKA); A8YER2 (MTNW_MICAE); [ Recover all ]
Triggered by HAMAP; MF_01679 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MTNW
Protein name RecName: Full=2,3-diketo-5-methylthiopentyl-1-phosphate enolase;
                 Short=DK-MTP-1-P enolase;
                 EC=5.3.2.5;
AltName: Full=RuBisCO-like protein;
                 Short=RLP;
Gene name Name=mtnW;

Comments [?]

FUNCTIONCatalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1- phosphate (HK-MTPenyl-1-P).
CATALYTIC ACTIVITY Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate = 2-hydroxy-5- methylsulfanyl-3-oxopent-1-enyl phosphate; Xref=Rhea:RHEA:18769, ChEBI:CHEBI:58828, ChEBI:CHEBI:59505; EC=5.3.2.5;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
SUBUNITHomodimer.
MISCELLANEOUSHas no RuBP-carboxylation activity.
SIMILARITYBelongs to the RuBisCO large chain family. Type IV subfamily.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0043715; Molecular function:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity
GO:0019284; Biological process:L-methionine salvage from S-adenosylmethionine

Cross-references [?]

PROSITE PS00157; RUBISCO_LARGE; 1;
Pfam PF00016; RuBisCO_large; 1;
NCBIfam TIGR03332; Salvage_mtnW; 1;

Features [?]

From: MTNW_GEOKA (Q5L1E2)
Key From To Description Tag Condition FTGroup
BINDING 173 176 /ligand="substrate"
BINDING 359 360 /ligand="substrate"
ACT_SITE 98 98 /note="Proton acceptor" K
BINDING 173 173 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/note="via carbamate group"
K
BINDING 175 175 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D
BINDING 176 176 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
E
BINDING 147 147 /ligand="substrate" K
BINDING 264 264 /ligand="substrate" H
BINDING 337 337 /ligand="substrate" G
MOD_RES 173 173 /note="N6-carboxylysine" K

Additional information [?]

Size range 374-414 amino acids
Related rules None
Fusion Nter: None Cter: None



View rule in raw text format (no links)