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HAMAP rule MF_01953

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General rule information [?]

Accession MF_01953
Dates 10-APR-2006 (Created)
7-MAY-2024 (Last updated, Version 37)
Name Urease_alpha
Scope(s) Bacteria
Archaea
Sulfolobales
Halobacteria
Template(s) P18314 (URE1_KLEAE); P77837 (URE1_BACSU); P69996 (URE1_HELPY); [ Recover all ]
Triggered by HAMAP; MF_01953 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier URE1
case not <OC:Campylobacterales>
Protein name RecName: Full=Urease subunit alpha;
                 EC=3.5.1.5;
AltName: Full=Urea amidohydrolase subunit alpha;
Gene name Name=ureC;
else case <OC:Campylobacterales>
Protein name RecName: Full=Urease subunit beta;
                 EC=3.5.1.5;
AltName: Full=Urea amidohydrolase subunit beta;
Gene name Name=ureB;
end case

Comments [?]

CATALYTIC ACTIVITY Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
case <FTGroup:1> and <FTGroup:2>
COFACTOR Name=Ni cation; Xref=ChEBI:CHEBI:25516; Note=Binds 2 nickel ions per subunit.;
end case
PATHWAYNitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.
case <OC:Campylobacterales>
SUBUNITHeterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits.
else case <OC:Deinococcus> or <OC:Sulfolobus>
SUBUNITHeterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits.
else case <OS:Pseudomonas syringae> or <OC:Streptomyces>
SUBUNITMay form a heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits. May also form a heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
else
SUBUNITHeterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
end case
SUBCELLULAR LOCATIONCytoplasm.
case <FT:4>
PTMCarboxylation allows a single lysine to coordinate two nickel ions.
end case
SIMILARITYBelongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
case <OC:Campylobacterales>
CAUTIONThe orthologous protein is known as the alpha subunit (UreC) in most other bacteria.
end case

Keywords [?]

Cytoplasm
Hydrolase
case <FTGroup:1> or <FTGroup:2>
Metal-binding
Nickel
end case

Gene Ontology [?]

GO:0009039; Molecular function:urease activity
case <FTGroup:1> or <FTGroup:2>
GO:0016151; Molecular function:nickel cation binding
end case
GO:0043419; Biological process:urea catabolic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS01120; UREASE_1; 1;
PROSITE PS00145; UREASE_2; 1;
PROSITE PS51368; UREASE_3; 1;
Pfam PF01979; Amidohydro_1; 1;
Pfam PF00449; Urease_alpha; 1;
PRINTS PR01752; UREASE; 1;
NCBIfam TIGR01792; Urease_alph; 1;

Features [?]

From: URE1_KLEAE (P18314)
Key From To Description Tag Condition FTGroup
ACT_SITE 320 320 /note="Proton donor" H
BINDING 134 134 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="1"
H 2
BINDING 136 136 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="1"
H 2
BINDING 217 217 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="1"
/note="via carbamate group"
K 1
BINDING 217 217 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="2"
/note="via carbamate group"
K 2
BINDING 246 246 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="2"
H 1
BINDING 272 272 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="2"
H 1
BINDING 360 360 /ligand="Ni(2+)"
/ligand_id="ChEBI:CHEBI:49786"
/ligand_label="1"
D 2
BINDING 219 219 /ligand="substrate" H
MOD_RES 217 217 /note="N6-carboxylysine" K

Additional information [?]

Size range 556-598 amino acids
Related rules None
Fusion Nter: None Cter: None



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