HAMAP rule MF_01953

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01953
Accession	MF_01953
Dates	10-APR-2006 (Created) 03-SEP-2024 (Last updated, Version 40)
Name	Urease_alpha
Scope(s)	Bacteria Archaea Sulfolobales Halobacteria
Template(s)	P18314; P77837; P69996; [ Recover all ]
Triggered by	HAMAP; MF_01953 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	URE1
case not <OC:Campylobacterales>
Protein name	RecName: Full=Urease subunit alpha; EC=3.5.1.5; AltName: Full=Urea amidohydrolase subunit alpha;
Gene name	Name=ureC;
else case <OC:Campylobacterales>
Protein name	RecName: Full=Urease subunit beta; EC=3.5.1.5; AltName: Full=Urea amidohydrolase subunit beta;
Gene name	Name=ureB;
end case

Comments [?]

CATALYTIC ACTIVITY	Reaction=urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+); Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, ChEBI:CHEBI:17544, ChEBI:CHEBI:28938; EC=3.5.1.5;
case <FTGroup:1> and <FTGroup:2>
COFACTOR	Name=Ni cation; Xref=ChEBI:CHEBI:25516; Note=Binds 2 nickel ions per subunit.;
end case
PATHWAY	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.
case <OC:Campylobacterales>
SUBUNIT	Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits.
else case <OC:Deinococcus> or <OC:Sulfolobus>
SUBUNIT	Heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits.
else case <OS:Pseudomonas syringae> or <OC:Streptomyces>
SUBUNIT	May form a heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits. May also form a heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
else
SUBUNIT	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
end case
SUBCELLULAR LOCATION	Cytoplasm.
case <FT:4>
PTM	Carboxylation allows a single lysine to coordinate two nickel ions.
end case
SIMILARITY	Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
case <OC:Campylobacterales>
CAUTION	The orthologous protein is known as the alpha subunit (UreC) in most other bacteria.
end case

Keywords [?]

Gene Ontology [?]

GO:0009039; Molecular function:urease activity
case <FTGroup:1> or <FTGroup:2>
GO:0016151; Molecular function:nickel cation binding
end case
GO:0043419; Biological process:urea catabolic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: URE1_KLEAE (P18314)
Key	From	To	Description	Tag	Condition	FTGroup
ACT_SITE	320	320	/note="Proton donor"		H
BINDING	134	134	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="1"		H	2
BINDING	136	136	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="1"		H	2
BINDING	217	217	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="1" /note="via carbamate group"		K	1
BINDING	217	217	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="2" /note="via carbamate group"		K	2
BINDING	246	246	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="2"		H	1
BINDING	272	272	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="2"		H	1
BINDING	360	360	/ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="1"		D	2
BINDING	219	219	/ligand="substrate"		H
MOD_RES	217	217	/note="N6-carboxylysine"		K

Additional information [?]