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Annotation rule MF_01953
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General rule information [?]

Accession MF_01953
Dates 10-APR-2006 (Created)
19-NOV-2019 (Last updated, Version 33)
Name Urease_alpha
Scope
Bacteria
Archaea; Sulfolobales
Archaea; Halobacteria
Templates P18314 (URE1_KLEAE); P77837 (URE1_BACSU); P69996 (URE1_HELPY): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
URE1
case not <OC:Campylobacterales>
Protein name
RecName: Full=Urease subunit alpha;
EC 3.5.1.5;
AltName: Full=Urea amidohydrolase subunit alpha;
Gene name
ureC
else case <OC:Campylobacterales>
Protein name
RecName: Full=Urease subunit beta;
EC 3.5.1.5;
AltName: Full=Urea amidohydrolase subunit beta;
Gene name
ureB
end case

Comments [?]

Catalytic activity RHEA:20557: 2 H(+) + H2O + urea = CO2 + 2 NH4(+)
EC 3.5.1.5
case <FTGroup:1> and <FTGroup:2>
Cofactor Ni cation
Note: Binds 2 nickel ions per subunit.
end case
Pathway Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.
case <OC:Campylobacterales>
Subunit Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits.
else case <OC:Deinococcus> or <OC:Sulfolobus>
Subunit Heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits.
else case <OS:Pseudomonas syringae> or <OC:Streptomyces>
Subunit May form a heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta) subunits. May also form a heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
else
Subunit Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
end case
Subcellular location Cytoplasm.
case <FT:4>
Ptm Carboxylation allows a single lysine to coordinate two nickel ions.
end case
Similarity Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
case <OC:Campylobacterales>
Caution The orthologous protein is known as the alpha subunit (UreC) in most other bacteria.
end case

Keywords [?]

case <FTGroup:1> or <FTGroup:2>
end case

Gene Ontology [?]

GO:0009039; Molecular function: urease activity.
case <FTGroup:1> or <FTGroup:2>
GO:0016151; Molecular function: nickel cation binding.
end case
GO:0019627; Biological process: urea metabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01120; UREASE_1; 1;
PS00145; UREASE_2; 1;
PS51368; UREASE_3; 1; trigger=PRU00700;
Pfam PF01979; Amidohydro_1; 1;
PF00449; Urease_alpha; 1;
PRINTS PR01752; UREASE; 1;
TIGRFAMs TIGR01792; Urease_alph; 1;

Features [?]

From: URE1_KLEAE (P18314)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     320     320       Proton donor     H  
METAL     134     134       Nickel 1; via tele nitrogen     H   2
METAL     136     136       Nickel 1; via tele nitrogen     H   2
METAL     217     217       Nickel 1; via carbamate group     K   1
METAL     217     217       Nickel 2; via carbamate group     K   2
METAL     246     246       Nickel 2; via pros nitrogen     H   1
METAL     272     272       Nickel 2; via tele nitrogen     H   1
METAL     360     360       Nickel 1     D   2
BINDING     219     219       Substrate     H  
MOD_RES     217     217       N6-carboxylysine     K  

Additional information [?]

Size range 556-598 amino acids
Related rules None
Fusion None