HAMAP rule MF_02041

General rule information [?]

Accession	MF_02041
Dates	20-JUL-2015 (Created) 1-JUN-2023 (Last updated, Version 7)

Name	DusA_subfam

Scope

Bacteria

Templates

Q5SMC7 (DUSAL_THET8); P32695 (DUSA_ECOLI): [Recover all]

Triggered by

HAMAP; MF_02041 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Pseudomonadota>

Identifier

DUSA

Protein name

RecName:		Full=tRNA-dihydrouridine(20/20a) synthase;
		EC 1.3.1.-;
		EC 1.3.1.91;
AltName:		Full=U20-specific dihydrouridine synthase;
		Short=U20-specific Dus;
AltName:		Full=tRNA-dihydrouridine synthase A;

Gene name

dusA

else

Identifier

DUSAL

Protein name

RecName:		Full=tRNA-dihydrouridine(20/20a) synthase;
		EC 1.3.1.-;
		EC 1.3.1.91;
AltName:		Full=DusA-like U20-specific dihydrouridine synthase;
		Short=U20-specific Dus;

end case

Comments [?]

Function	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity	RHEA:53336: 5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA EC 1.3.1.91
	RHEA:53340: 5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA EC 1.3.1.91
	RHEA:53344: 5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA
	RHEA:53348: 5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA
Cofactor	FMN
Similarity	Belongs to the Dus family. DusA subfamily.

Keywords [?]

Flavoprotein
FMN
NADP
Oxidoreductase
RNA-binding
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE	PS01136; UPF0034; 1;
Pfam	PF01207; Dus; 1;
NCBIfam	TIGR00742; yjbN; 1;
PIRSF	PIRSF006621; Dus; 1;

Features [?]

From: DUSAL_THET8 (Q5SMC7)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 10 12 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 P-M-[VML]

BINDING (Optional) 203 205 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 N-G-G

BINDING (Optional) 225 226 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 G-R

ACT_SITE 93 93 Proton donor C

BINDING (Optional) 63 63 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 Q

BINDING (Optional) 132 132 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 K

BINDING (Optional) 164 164 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 H

SITE (Optional) 90 90 Interacts with tRNA N

SITE (Optional) 175 175 Interacts with tRNA; defines subfamily-specific binding signature K

SITE (Optional) 178 178 Interacts with tRNA R

SITE (Optional) 290 290 Interacts with tRNA; defines subfamily-specific binding signature R

SITE (Optional) 293 293 Interacts with tRNA; defines subfamily-specific binding signature R

Additional information [?]

Size range	288-400 amino acids
Related rules	None
Fusion	None

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