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Annotation rule MF_02041
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General rule information [?]

Accession MF_02041
Dates 20-JUL-2015 (Created)
20-NOV-2019 (Last updated, Version 3)
Name DusA_subfam
Scope
Bacteria
Templates Q5SMC7 (DUSAL_THET8); P32695 (DUSA_ECOLI): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Proteobacteria>
Identifier
DUSA
Protein name
RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
EC 1.3.1.-;
EC 1.3.1.91;
AltName: Full=U20-specific dihydrouridine synthase;
Short=U20-specific Dus;
AltName: Full=tRNA-dihydrouridine synthase A;
Gene name
dusA
else
Identifier
DUSAL
Protein name
RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
EC 1.3.1.-;
EC 1.3.1.91;
AltName: Full=DusA-like U20-specific dihydrouridine synthase;
Short=U20-specific Dus;
end case

Comments [?]

Function Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity RHEA:53336: 5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA
EC 1.3.1.91
RHEA:53340: 5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA
EC 1.3.1.91
RHEA:53344: 5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA
RHEA:53348: 5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA
Cofactor FMN
Similarity Belongs to the Dus family. DusA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
TIGRFAMs TIGR00742; yjbN; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

From: DUSAL_THET8 (Q5SMC7)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     10     12       FMN     P-M-[VML]  
NP_BIND (Optional)     203     205       FMN     N-G-G  
NP_BIND (Optional)     225     226       FMN     G-R  
ACT_SITE     93     93       Proton donor     C  
BINDING (Optional)     63     63       FMN     Q  
BINDING (Optional)     132     132       FMN     K  
BINDING (Optional)     164     164       FMN     H  
SITE (Optional)     90     90       Interacts with tRNA     N  
SITE (Optional)     175     175       Interacts with tRNA; defines subfamily-specific binding signature     K  
SITE (Optional)     178     178       Interacts with tRNA     R  
SITE (Optional)     290     290       Interacts with tRNA; defines subfamily-specific binding signature     R  
SITE (Optional)     293     293       Interacts with tRNA; defines subfamily-specific binding signature     R  

Additional information [?]

Size range 288-400 amino acids
Related rules None
Fusion None