HAMAP rule MF_02043

General rule information [?]

Accession	MF_02043
Dates	20-JUL-2015 (Created) 26-JAN-2023 (Last updated, Version 6)
Name	DusC_subfam
Scope(s)	Bacteria Pseudomonadota
Template(s)	P33371 (DUSC_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_02043 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	DUSC
Protein name	RecName: Full=tRNA-dihydrouridine(16) synthase; EC=1.3.1.-; AltName: Full=U16-specific dihydrouridine synthase; Short=U16-specific Dus; AltName: Full=tRNA-dihydrouridine synthase C;
Gene name	Name=dusC;

Comments [?]

FUNCTION	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
CATALYTIC ACTIVITY	Reaction=5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA; Xref=Rhea:RHEA:53376, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CATALYTIC ACTIVITY	Reaction=5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA; Xref=Rhea:RHEA:53380, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
COFACTOR	Name=FMN; Xref=ChEBI:CHEBI:58210;
SIMILARITY	Belongs to the Dus family. DusC subfamily.

Keywords [?]

Gene Ontology [?]

GO:0010181; Molecular function:FMN binding

GO:0000049; Molecular function:tRNA binding

GO:0017150; Molecular function:tRNA dihydrouridine synthase activity

GO:0002943; Biological process:tRNA dihydrouridine synthesis

Cross-references [?]

Features [?]

From: DUSC_ECOLI (P33371)

Key

From

Description

Tag

Condition

FTGroup

BINDING