HAMAP rule MF_02043

General rule information [?]

Accession	MF_02043
Dates	20-JUL-2015 (Created) 26-JAN-2023 (Last updated, Version 6)

Name	DusC_subfam

Scope

Bacteria; Pseudomonadota

Template

P33371 (DUSC_ECOLI)

Triggered by

HAMAP; MF_02043 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DUSC

Protein name

RecName:		Full=tRNA-dihydrouridine(16) synthase;
		EC 1.3.1.-;
AltName:		Full=U16-specific dihydrouridine synthase;
		Short=U16-specific Dus;
AltName:		Full=tRNA-dihydrouridine synthase C;

Gene name

dusC

Comments [?]

Function	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
Catalytic activity	RHEA:53376: 5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA
	RHEA:53380: 5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA
Cofactor	FMN
Similarity	Belongs to the Dus family. DusC subfamily.

Keywords [?]

Flavoprotein
FMN
NADP
Oxidoreductase
RNA-binding
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE	PS01136; UPF0034; 1;
Pfam	PF01207; Dus; 1;
PIRSF	PIRSF006621; Dus; 1;

Features [?]

From: DUSC_ECOLI (P33371)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 7 9 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 P-M-[EQ]

BINDING (Optional) 200 202 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 N-G-[ED]

BINDING (Optional) 224 225 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [GC]-[RC]

ACT_SITE 98 98 Proton donor C

BINDING (Optional) 68 68 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 Q

BINDING (Optional) 139 139 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [KR]

SITE (Optional) 35 35 Interacts with tRNA; defines subfamily-specific binding signature R

SITE (Optional) 95 95 Interacts with tRNA N

SITE (Optional) 176 176 Interacts with tRNA Y

SITE (Optional) 272 272 Interacts with tRNA; defines subfamily-specific binding signature R

SITE (Optional) 274 274 Interacts with tRNA; defines subfamily-specific binding signature K

SITE (Optional) 279 279 Interacts with tRNA Y

SITE (Optional) 295 295 Interacts with tRNA; defines subfamily-specific binding signature [RK]

Additional information [?]

Size range	290-360 amino acids
Related rules	None
Fusion	None

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