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Annotation rule MF_02043 |
Accession | MF_02043 |
Dates | 20-JUL-2015 (Created) 20-NOV-2019 (Last updated, Version 3) |
Name | DusC_subfam |
Scope | Bacteria; Proteobacteria |
Template | P33371 (DUSC_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. |
Catalytic activity | RHEA:53376: 5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA |
RHEA:53380: 5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA | |
Cofactor | FMN |
Similarity | Belongs to the Dus family. DusC subfamily. |
PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
PIRSF | PIRSF006621; Dus; 1; |
From: DUSC_ECOLI (P33371) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND (Optional) | 7 | 9 | FMN | P-M-[EQ] | ||||||||
NP_BIND (Optional) | 200 | 202 | FMN | N-G-[ED] | ||||||||
NP_BIND (Optional) | 224 | 225 | FMN | [GC]-[RC] | ||||||||
ACT_SITE | 98 | 98 | Proton donor | C | ||||||||
BINDING (Optional) | 68 | 68 | FMN | Q | ||||||||
BINDING (Optional) | 139 | 139 | FMN | [KR] | ||||||||
SITE (Optional) | 35 | 35 | Interacts with tRNA; defines subfamily-specific binding signature | R | ||||||||
SITE (Optional) | 95 | 95 | Interacts with tRNA | N | ||||||||
SITE (Optional) | 176 | 176 | Interacts with tRNA | Y | ||||||||
SITE (Optional) | 272 | 272 | Interacts with tRNA; defines subfamily-specific binding signature | R | ||||||||
SITE (Optional) | 274 | 274 | Interacts with tRNA; defines subfamily-specific binding signature | K | ||||||||
SITE (Optional) | 279 | 279 | Interacts with tRNA | Y | ||||||||
SITE (Optional) | 295 | 295 | Interacts with tRNA; defines subfamily-specific binding signature | [RK] |
Size range | 290-360 amino acids |
Related rules | None |
Fusion | None |