HAMAP rule MF_02043
General rule information
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Accession | MF_02043 |
Dates | 20-JUL-2015 (Created)
26-JAN-2023 (Last updated, Version 6) |
Name | DusC_subfam |
Scope(s) |
Bacteria Pseudomonadota |
Template(s) | P33371 (DUSC_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_02043 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | DUSC |
Protein name | RecName: Full=tRNA-dihydrouridine(16) synthase; EC=1.3.1.-; AltName: Full=U16-specific dihydrouridine synthase; Short=U16-specific Dus; AltName: Full=tRNA-dihydrouridine synthase C; |
Gene name | Name=dusC; |
Comments
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FUNCTION | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA; Xref=Rhea:RHEA:53376, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA; Xref=Rhea:RHEA:53380, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
SIMILARITY | Belongs to the Dus family. DusC subfamily. |
Keywords
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Gene Ontology
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GO:0010181; Molecular function:FMN binding |
GO:0000049; Molecular function:tRNA binding |
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity |
GO:0002943; Biological process:tRNA dihydrouridine synthesis |
Cross-references
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PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
PIRSF | PIRSF006621; Dus; 1; |
Features
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From: DUSC_ECOLI (P33371) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 7 | 9 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
P-M-[EQ] | ||||||||
BINDING | 200 | 202 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
N-G-[ED] | ||||||||
BINDING | 224 | 225 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[GC]-[RC] | ||||||||
ACT_SITE | 98 | 98 | /note="Proton donor" | C | ||||||||
BINDING | 68 | 68 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q | ||||||||
BINDING | 139 | 139 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[KR] | ||||||||
SITE | 35 | 35 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | R | ||||||||
SITE | 95 | 95 | /note="Interacts with tRNA" | N | ||||||||
SITE | 176 | 176 | /note="Interacts with tRNA" | Y | ||||||||
SITE | 272 | 272 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | R | ||||||||
SITE | 274 | 274 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | K | ||||||||
SITE | 279 | 279 | /note="Interacts with tRNA" | Y | ||||||||
SITE | 295 | 295 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | [RK] |
Additional information
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Size range | 290-360 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |