HAMAP rule MF_02043
General rule information
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Accession | MF_02043 |
Dates | 20-JUL-2015 (Created) 26-JAN-2023 (Last updated, Version 6) |
Name | DusC_subfam |
Scope | Bacteria; Pseudomonadota |
Template | P33371 (DUSC_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. |
Catalytic activity | RHEA:53376: 5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA |
RHEA:53380: 5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA | |
Cofactor | FMN |
Similarity | Belongs to the Dus family. DusC subfamily. |
Keywords
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Gene Ontology
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GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.
Cross-references
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PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
PIRSF | PIRSF006621; Dus; 1; |
Features
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From: DUSC_ECOLI (P33371) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING (Optional) | 7 | 9 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | P-M-[EQ] | ||||||||
BINDING (Optional) | 200 | 202 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | N-G-[ED] | ||||||||
BINDING (Optional) | 224 | 225 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | [GC]-[RC] | ||||||||
ACT_SITE | 98 | 98 | Proton donor | C | ||||||||
BINDING (Optional) | 68 | 68 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | Q | ||||||||
BINDING (Optional) | 139 | 139 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | [KR] | ||||||||
SITE (Optional) | 35 | 35 | Interacts with tRNA; defines subfamily-specific binding signature | R | ||||||||
SITE (Optional) | 95 | 95 | Interacts with tRNA | N | ||||||||
SITE (Optional) | 176 | 176 | Interacts with tRNA | Y | ||||||||
SITE (Optional) | 272 | 272 | Interacts with tRNA; defines subfamily-specific binding signature | R | ||||||||
SITE (Optional) | 274 | 274 | Interacts with tRNA; defines subfamily-specific binding signature | K | ||||||||
SITE (Optional) | 279 | 279 | Interacts with tRNA | Y | ||||||||
SITE (Optional) | 295 | 295 | Interacts with tRNA; defines subfamily-specific binding signature | [RK] |
Additional information
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Size range | 290-360 amino acids |
Related rules | None |
Fusion | None |