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Annotation rule MF_02043
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General rule information [?]

Accession MF_02043
Dates 20-JUL-2015 (Created)
20-NOV-2019 (Last updated, Version 3)
Name DusC_subfam
Scope
Bacteria; Proteobacteria
Template P33371 (DUSC_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DUSC
Protein name
RecName: Full=tRNA-dihydrouridine(16) synthase;
EC 1.3.1.-;
AltName: Full=U16-specific dihydrouridine synthase;
Short=U16-specific Dus;
AltName: Full=tRNA-dihydrouridine synthase C;
Gene name
dusC

Comments [?]

Function Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
Catalytic activity RHEA:53376: 5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA
RHEA:53380: 5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA
Cofactor FMN
Similarity Belongs to the Dus family. DusC subfamily.

Keywords [?]

Flavoprotein
FMN
NADP
Oxidoreductase
RNA-binding
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0010181; Molecular function: FMN binding.
GO:0000049; Molecular function: tRNA binding.
GO:0017150; Molecular function: tRNA dihydrouridine synthase activity.
GO:0002943; Biological process: tRNA dihydrouridine synthesis.

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

From: DUSC_ECOLI (P33371)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     7     9       FMN     P-M-[EQ]  
NP_BIND (Optional)     200     202       FMN     N-G-[ED]  
NP_BIND (Optional)     224     225       FMN     [GC]-[RC]  
ACT_SITE     98     98       Proton donor     C  
BINDING (Optional)     68     68       FMN     Q  
BINDING (Optional)     139     139       FMN     [KR]  
SITE (Optional)     35     35       Interacts with tRNA; defines subfamily-specific binding signature     R  
SITE (Optional)     95     95       Interacts with tRNA     N  
SITE (Optional)     176     176       Interacts with tRNA     Y  
SITE (Optional)     272     272       Interacts with tRNA; defines subfamily-specific binding signature     R  
SITE (Optional)     274     274       Interacts with tRNA; defines subfamily-specific binding signature     K  
SITE (Optional)     279     279       Interacts with tRNA     Y  
SITE (Optional)     295     295       Interacts with tRNA; defines subfamily-specific binding signature     [RK]  

Additional information [?]

Size range 290-360 amino acids
Related rules None
Fusion None


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