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HAMAP rule MF_02084

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General rule information [?]

Accession MF_02084
Dates 5-JAN-2017 (Created)
3-SEP-2024 (Last updated, Version 13)
Name LysJ_aminotrans_3
Scope(s) Archaea
Bacteria
Chloroflexota
Deinococcota
Template(s) Q4JAP8 (LYSJ_SULAC); Q93R93 (LYSJ_THET2); Q5SHH5 (LYSJ_THET8); [ Recover all ]
Triggered by HAMAP; MF_02084 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LYSJ
case <OC:Sulfolobales>
Protein name RecName: Full=[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase;
                 EC=2.6.1.118;
                 EC=2.6.1.124;
else case <OC:Archaea> and not <OC:Sulfolobales>
Protein name RecName: Full=Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase;
                 EC=2.6.1.118;
                 EC=2.6.1.124;
else case <OC:Deinococcota>
Protein name RecName: Full=[LysW]-aminoadipate semialdehyde transaminase;
                 EC=2.6.1.118;
else case <OC:Chloroflexota>
Protein name RecName: Full=Putative [LysW]-aminoadipate semialdehyde transaminase;
                 EC=2.6.1.118;
end case
Gene name Name=lysJ;

Comments [?]

case <OC:Archaea>
FUNCTIONInvolved in both the arginine and lysine biosynthetic pathways.
else case <OC:Bacteria>
FUNCTIONCatalyzes the transfer of the amino group of L-glutamate to [LysW]-aminoadipate 6-semialdehyde, generating [LysW]-gamma-L-lysine.
end case
CATALYTIC ACTIVITY Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L- glutamate + 2-oxoglutarate = [amino-group carrier protein]-C- terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate; Xref=Rhea:RHEA:41952, Rhea:RHEA-COMP:9714, Rhea:RHEA-COMP:9715, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:78501, ChEBI:CHEBI:78526; EC=2.6.1.118;
case <OC:Archaea>
CATALYTIC ACTIVITY Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L- glutamate + 2-oxoglutarate = [amino-group carrier protein]-C- terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate; Xref=Rhea:RHEA:52672, Rhea:RHEA-COMP:13327, Rhea:RHEA-COMP:13328, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:136761, ChEBI:CHEBI:136763; EC=2.6.1.124;
end case
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Note=Binds 1 pyridoxal phosphate per subunit
PATHWAYAmino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 4/5.
case <OC:Archaea>
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis.
end case
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.

Keywords [?]

Amino-acid biosynthesis
Aminotransferase
case <OC:Archaea>
Arginine biosynthesis
end case
Cytoplasm
Lysine biosynthesis
Pyridoxal phosphate
Transferase

Gene Ontology [?]

GO:0008483; Molecular function:transaminase activity
case <OCellular component:Archaea>
GO:0042450; Biological process:arginine biosynthetic process via ornithine
end case
GO:0019878; Biological process:lysine biosynthetic process via aminoadipic acid
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS00600; AA_TRANSFER_CLASS_3; 1;
Pfam PF00202; Aminotran_3; 1;
NCBIfam TIGR00707; argD; 1;

Features [?]

From: LYSJ_THET8 (Q5SHH5)
Key From To Description Tag Condition FTGroup
BINDING 113 114 /ligand="pyridoxal 5'-phosphate"
/ligand_id="ChEBI:CHEBI:597326"		 G-[TA]
BINDING 225 228 /ligand="pyridoxal 5'-phosphate"
/ligand_id="ChEBI:CHEBI:597326"		 D-E-[IV]-Q
BINDING 140 140 /ligand="pyridoxal 5'-phosphate"
/ligand_id="ChEBI:CHEBI:597326"		 F
BINDING 143 143 /ligand="substrate" R
BINDING 282 282 /ligand="substrate" [ST]
BINDING 283 283 /ligand="pyridoxal 5'-phosphate"
/ligand_id="ChEBI:CHEBI:597326"		 T
MOD_RES 254 254 /note="N6-(pyridoxal phosphate)lysine" K

Additional information [?]

Size range 360-450 amino acids
Related rules None
Fusion Nter: None Cter: None



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