HAMAP rule MF_02090
General rule information
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| Accession | MF_02090 |
| Dates | 29-MAR-2017 (Created)
1-JUN-2023 (Last updated, Version 8) |
| Name | NadE_glutamine_dep |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P9WJJ3 (NADE_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_02090 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NADE |
| Protein name | RecName: Full=Glutamine-dependent NAD(+) synthetase; EC=6.3.5.1; AltName: Full=NAD(+) synthase [glutamine-hydrolyzing]; |
| Gene name | Name=nadE; |
Comments
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| FUNCTION | Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. |
| CATALYTIC ACTIVITY | Reaction=ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+); Xref=Rhea:RHEA:24384, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58359, ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.5.1; |
| PATHWAY | Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1. |
| SIMILARITY | In the C-terminal section; belongs to the NAD synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0008795; Molecular function:NAD+ synthase activity |
| GO:0009435; Biological process:NAD biosynthetic process |
Cross-references
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| PROSITE | PS50263; CN_HYDROLASE; 1; |
| Pfam | PF00795; CN_hydrolase; 1; |
| Pfam | PF02540; NAD_synthase; 1; |
| NCBIfam | TIGR00552; nadE; 1; |
| PIRSF | PIRSF006630; NADS_GAT; 1; |
Features
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| From: NADE_MYCTU (P9WJJ3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 366 | 373 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GAP]-x-S-G-G-x-D-S | ||||||||
| BINDING | 490 | 493 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" |
W-x-T-[YF] | ||||||||
| ACT_SITE | 52 | 52 | /note="Proton acceptor; for glutaminase activity" | E | ||||||||
| ACT_SITE | 121 | 121 | /note="For glutaminase activity" | K | ||||||||
| ACT_SITE | 176 | 176 | /note="Nucleophile; for glutaminase activity" | C | ||||||||
| BINDING | 127 | 127 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
Y | ||||||||
| BINDING | 203 | 203 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
S | ||||||||
| BINDING | 209 | 209 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
[RK] | ||||||||
| BINDING | 456 | 456 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" |
N | ||||||||
| BINDING | 480 | 480 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 485 | 485 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" |
E | ||||||||
| BINDING | 635 | 635 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" |
K | ||||||||
Additional information
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| Size range | 510-735 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |