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Annotation rule MF_02090
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General rule information [?]

Accession MF_02090
Dates 29-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 4)
Name NadE_glutamine_dep
Scope
Bacteria
Archaea
Template P9WJJ3 (NADE_MYCTU)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NADE
Protein name
RecName: Full=Glutamine-dependent NAD(+) synthetase;
EC 6.3.5.1;
AltName: Full=NAD(+) synthase [glutamine-hydrolyzing];
Gene name
nadE

Comments [?]

Function Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.
Catalytic activity RHEA:24384: ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)
EC 6.3.5.1
Pathway Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.
Similarity In the C-terminal section; belongs to the NAD synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0008795; Molecular function: NAD+ synthase activity.
GO:0009435; Biological process: NAD biosynthetic process.

Cross-references [?]

PROSITE PS50263; CN_HYDROLASE; 1; trigger=PRU00054;
Pfam PF00795; CN_hydrolase; 1;
PF02540; NAD_synthase; 1;
TIGRFAMs TIGR00552; nadE; 1;
PIRSF PIRSF006630; NADS_GAT; 1;

Features [?]

From: NADE_MYCTU (P9WJJ3)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     366     373       ATP     [GAP]-x-S-G-G-x-D-S  
NP_BIND (Optional)     490     493       Deamido-NAD     W-x-T-[YF]  
ACT_SITE     52     52       Proton acceptor; for glutaminase activity     E  
ACT_SITE     121     121       For glutaminase activity     K  
ACT_SITE     176     176       Nucleophile; for glutaminase activity     C  
BINDING (Optional)     127     127       L-glutamine     Y  
BINDING (Optional)     203     203       L-glutamine     S  
BINDING (Optional)     209     209       L-glutamine     [RK]  
BINDING (Optional)     456     456       Deamido-NAD     N  
BINDING (Optional)     480     480       ATP     T  
BINDING (Optional)     485     485       Deamido-NAD     E  
BINDING (Optional)     635     635       Deamido-NAD     K  

Additional information [?]

Size range 510-735 amino acids
Related rules None
Fusion None