HAMAP rule MF_02234
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02234 |
| Accession | MF_02234 |
| Dates | 15-OCT-2019 (Created)
5-NOV-2024 (Last updated, Version 9) |
| Name | AOTCase |
| Scope(s) |
Bacteria |
| Template(s) | Q8P8J2 (AOTC_XANCP); [ Recover all ] |
| Triggered by |
HAMAP; MF_02234 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | AOTC |
| Protein name | RecName: Full=N-acetylornithine carbamoyltransferase; EC=2.1.3.9; AltName: Full=N-acetyl-L-ornithine transcarbamylase; Short=AOTCase; Short=Acetylornithine transcarbamylase; |
| Gene name | Name=argF'; |
Comments
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| FUNCTION | Catalyzes the transfer of the carbamoyl group from carbamoyl phosphate to the delta-amino group of N(2)-acetyl-L-ornithine to produce N(2)-acetyl-L-citrulline. This is a step in an alternative arginine biosynthesis pathway. |
| CATALYTIC ACTIVITY | Reaction=N(2)-acetyl-L-ornithine + carbamoyl phosphate = N(2)-acetyl-L- citrulline + phosphate + H(+); Xref=Rhea:RHEA:18609, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57805, ChEBI:CHEBI:58228, ChEBI:CHEBI:58765; EC=2.1.3.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18610; |
| PATHWAY | Amino-acid biosynthesis; L-arginine biosynthesis. |
| SUBUNIT | Homotrimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the aspartate/ornithine carbamoyltransferase superfamily. AOTCase family. |
Keywords
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Gene Ontology
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| GO:0043857; Molecular function:N-acetylornithine carbamoyltransferase activity |
| GO:0006526; Biological process:L-arginine biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: AOTC_XANCP (Q8P8J2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 49 | 52 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
S-x-R-T | ||||||||
| BINDING | 148 | 151 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
H-P-C-Q | ||||||||
| BINDING | 294 | 295 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
C-L | ||||||||
| BINDING | 77 | 77 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" |
W | ||||||||
| BINDING | 112 | 112 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
R | ||||||||
| BINDING | 144 | 144 | /ligand="N(2)-acetyl-L-ornithine" /ligand_id="ChEBI:CHEBI:57805" |
E | ||||||||
| BINDING | 252 | 252 | /ligand="N(2)-acetyl-L-ornithine" /ligand_id="ChEBI:CHEBI:57805" |
K | ||||||||
| BINDING | 295 | 295 | /ligand="N(2)-acetyl-L-ornithine" /ligand_id="ChEBI:CHEBI:57805" |
L | ||||||||
| BINDING | 322 | 322 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
R | ||||||||
| SITE | 92 | 92 | /note="Key residue in conferring substrate specificity for N-acetyl-L-ornithine versus N-succinyl-L-ornithine" | E | ||||||||
| MOD_RES | 302 | 302 | /note="N6-carboxylysine" | K | ||||||||
Additional information
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| Size range | 310-370 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |