HAMAP rule MF_03038
General rule information
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Accession | MF_03038 |
Dates | 20-JUL-2009 (Created) 19-NOV-2022 (Last updated, Version 12) |
Name | NUBP1 |
Scope | Eukaryota |
Templates | P53384 (NUBP1_HUMAN); P52920 (NBP35_YEAST); Q9R060 (NUBP1_MOUSE); Q8H1Q2 (NBP35_ARATH): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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case <OC:Fungi> or <OC:Viridiplantae>
Identifier |
|
else
Identifier |
|
end case
case <OC:Mammalia>
Protein name |
|
else case <OC:Vertebrata>
Protein name |
|
else case <OC:Fungi>
Protein name |
|
else case <OC:Viridiplantae>
Protein name |
|
else
Protein name |
|
end case
case <OC:Vertebrata>
Gene name |
|
else case <OC:Fungi> or <OC:Viridiplantae>
Gene name |
|
end case
Comments
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case <OC:Saccharomycetes>
Function | Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export. |
else case <OC:Fungi>
Function | Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. |
else case <OC:Viridiplantae>
Function | Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development. |
else case <OC:Mammalia>
Function | Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. |
else
Function | Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. |
end case
case <OC:Viridiplantae>
Cofactor | [4Fe-4S] cluster Note: Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable clusters in the N-termini and one labile, bridging cluster between subunits of the homodimer. |
else case <OC:Fungi>
Cofactor | [4Fe-4S] cluster Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NBP35) and two labile, bridging clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer. |
else
Cofactor | [4Fe-4S] cluster Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NUBP1) and two labile, bridging clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer. |
end case
case <OC:Viridiplantae>
Subunit | Homodimer and homotetramer. Predominantly homodimeric. |
else case <OC:Mammalia>
Subunit | Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. |
else case <OC:Fungi>
Subunit | Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains. |
else
Subunit | Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains. |
end case
case <OC:Saccharomycetes>
Subcellular location | Cytoplasm. Nucleus. |
else
Subcellular location | Cytoplasm. |
end case
Similarity | Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily. |
case <OC:Viridiplantae>
Miscellaneous | Although plant and algal NBP35 proteins lack the characteristic CXXC motif in the C-terminus, thought to be required for Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C-terminus. Also, in this linage, no CFD1 partner protein homolog as found in other eukaryotes can be found. |
end case
Keywords
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case <FTGroup:1> or <FTGroup:2>
end case
case <FT:1>
end case
case <OC:Saccharomycetes>
end case
Gene Ontology
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GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0016226; Biological process: iron-sulfur cluster assembly.
GO:0005737; Cellular component: cytoplasm.
GO:0016226; Biological process: iron-sulfur cluster assembly.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Features
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From: NBP35_YEAST (P52920) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 80 | 87 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-K-G-G-V-G-K-S | ||||||||
BINDING | 27 | 27 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 | C | 1 | |||||||
BINDING | 41 | 41 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 | C | 1 | |||||||
BINDING | 44 | 44 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 | C | 1 | |||||||
BINDING | 50 | 50 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 | C | 1 |
case not <OC:Viridiplantae>
end case
Additional information
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Size range | 310-345 amino acids |
Related rules | MF_02040 (APBC) |
Fusion | None |