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Annotation rule MF_03038
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General rule information [?]

Accession MF_03038
Dates 20-JUL-2009 (Created)
19-NOV-2022 (Last updated, Version 12)
Name NUBP1
Scope
Eukaryota
Templates P53384 (NUBP1_HUMAN); P52920 (NBP35_YEAST); Q9R060 (NUBP1_MOUSE); Q8H1Q2 (NBP35_ARATH): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Fungi> or <OC:Viridiplantae>
Identifier
NBP35
else
Identifier
NUBP1
end case
case <OC:Mammalia>
Protein name
RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP1;
AltName: Full=Nucleotide-binding protein 1;
Short=NBP 1;
else case <OC:Vertebrata>
Protein name
RecName: Full=Cytosolic Fe-S cluster assembly factor ;
AltName: Full=Nucleotide-binding protein 1;
Short=NBP 1;
else case <OC:Fungi>
Protein name
RecName: Full=Cytosolic Fe-S cluster assembly factor ;
AltName: Full=Nucleotide-binding protein 35;
else case <OC:Viridiplantae>
Protein name
RecName: Full=Cytosolic Fe-S cluster assembly factor NBP35;
else
Protein name
RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP1 homolog;
end case
case <OC:Vertebrata>
Gene name
NUBP1
else case <OC:Fungi> or <OC:Viridiplantae>
Gene name
NBP35
end case

Comments [?]

case <OC:Saccharomycetes>
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.
else case <OC:Fungi>
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.
else case <OC:Viridiplantae>
Function Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development.
else case <OC:Mammalia>
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication.
else
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.
end case
case <OC:Viridiplantae>
Cofactor [4Fe-4S] cluster
Note: Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable clusters in the N-termini and one labile, bridging cluster between subunits of the homodimer.
else case <OC:Fungi>
Cofactor [4Fe-4S] cluster
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NBP35) and two labile, bridging clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer.
else
Cofactor [4Fe-4S] cluster
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NUBP1) and two labile, bridging clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer.
end case
case <OC:Viridiplantae>
Subunit Homodimer and homotetramer. Predominantly homodimeric.
else case <OC:Mammalia>
Subunit Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1.
else case <OC:Fungi>
Subunit Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains.
else
Subunit Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains.
end case
case <OC:Saccharomycetes>
Subcellular location Cytoplasm. Nucleus.
else
Subcellular location Cytoplasm.
end case
Similarity Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.
case <OC:Viridiplantae>
Miscellaneous Although plant and algal NBP35 proteins lack the characteristic CXXC motif in the C-terminus, thought to be required for Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C-terminus. Also, in this linage, no CFD1 partner protein homolog as found in other eukaryotes can be found.
end case

Keywords [?]

case <FTGroup:1> or <FTGroup:2>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
case <FT:1>
ATP-binding
Nucleotide-binding
end case
Cytoplasm
case <OC:Saccharomycetes>
Nucleus
end case

Gene Ontology [?]

GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0016226; Biological process: iron-sulfur cluster assembly.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01215; MRP; 1;
Pfam PF10609; ParA; 1;

Features [?]

From: NBP35_YEAST (P52920)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     80     87       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-K-G-G-V-G-K-S  
BINDING     27     27       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C   1
BINDING     41     41       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C   1
BINDING     44     44       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C   1
BINDING     50     50       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C   1
case not <OC:Viridiplantae>
BINDING     253     253       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner     C   2
BINDING     256     256       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner     C   2
end case

Additional information [?]

Size range 310-345 amino acids
Related rules MF_02040 (APBC)
Fusion None


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