HAMAP rule MF_03038

General rule information [?]

Accession	MF_03038
Dates	20-JUL-2009 (Created) 19-NOV-2022 (Last updated, Version 12)

Name

NUBP1

Scope

Eukaryota

Templates

P53384 (NUBP1_HUMAN); P52920 (NBP35_YEAST); Q9R060 (NUBP1_MOUSE); Q8H1Q2 (NBP35_ARATH): [Recover all]

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03038 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Fungi> or <OC:Viridiplantae>

Identifier

NBP35

else

Identifier

NUBP1

end case

case <OC:Mammalia>

Protein name

RecName:		Full=Cytosolic Fe-S cluster assembly factor NUBP1;
AltName:		Full=Nucleotide-binding protein 1;
		Short=NBP 1;

else case <OC:Vertebrata>

Protein name

RecName:		Full=Cytosolic Fe-S cluster assembly factor ;
AltName:		Full=Nucleotide-binding protein 1;
		Short=NBP 1;

else case <OC:Fungi>

Protein name

RecName:		Full=Cytosolic Fe-S cluster assembly factor ;
AltName:		Full=Nucleotide-binding protein 35;

else case <OC:Viridiplantae>

Protein name

RecName:

Full=Cytosolic Fe-S cluster assembly factor NBP35;

else

Protein name

RecName:

Full=Cytosolic Fe-S cluster assembly factor NUBP1 homolog;

end case

case <OC:Vertebrata>

Gene name

NUBP1

else case <OC:Fungi> or <OC:Viridiplantae>

Gene name

NBP35

end case

Comments [?]

case <OC:Saccharomycetes>

Function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.

else case <OC:Fungi>

Function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.

else case <OC:Viridiplantae>

Function

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development.

else case <OC:Mammalia>

Function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication.

else

Function

end case

case <OC:Viridiplantae>

Cofactor

[4Fe-4S] cluster
Note: Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable clusters in the N-termini and one labile, bridging cluster between subunits of the homodimer.

else case <OC:Fungi>

Cofactor

[4Fe-4S] cluster
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NBP35) and two labile, bridging clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer.

else

Cofactor

[4Fe-4S] cluster
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NUBP1) and two labile, bridging clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer.

end case

case <OC:Viridiplantae>

Subunit

Homodimer and homotetramer. Predominantly homodimeric.

else case <OC:Mammalia>

Subunit

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1.

else case <OC:Fungi>

Subunit

Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains.

else

Subunit

Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains.

end case

case <OC:Saccharomycetes>

Subcellular location

Cytoplasm. Nucleus.

else

Subcellular location

Cytoplasm.

end case

Similarity

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.

case <OC:Viridiplantae>

Miscellaneous

Although plant and algal NBP35 proteins lack the characteristic CXXC motif in the C-terminus, thought to be required for Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C-terminus. Also, in this linage, no CFD1 partner protein homolog as found in other eukaryotes can be found.

end case

Keywords [?]

case <FTGroup:1> or <FTGroup:2>

4Fe-4S
Iron
Iron-sulfur
Metal-binding

end case

case <FT:1>

ATP-binding
Nucleotide-binding

end case

Cytoplasm

case <OC:Saccharomycetes>

Nucleus

end case

Gene Ontology [?]

GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0016226; Biological process: iron-sulfur cluster assembly.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS01215; MRP; 1;
Pfam	PF10609; ParA; 1;

Features [?]

From: NBP35_YEAST (P52920)

Key From To Description Tag Condition FTGroup

BINDING 80 87 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-K-G-G-V-G-K-S

BINDING 27 27 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 41 41 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 44 44 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 50 50 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

case not <OC:Viridiplantae>

BINDING 253 253 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner C 2

BINDING 256 256 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner C 2

end case

Additional information [?]

Size range	310-345 amino acids
Related rules	MF_02040 (APBC)
Fusion	None

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