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|
Annotation rule MF_03038 |
General rule information
[?]
Accession |
MF_03038 |
Dates |
20-JUL-2009 (Created) 19-NOV-2022 (Last updated, Version 12) |
case <OC:Eukaryota>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Fungi> or <OC:Viridiplantae>
else
end case
case <OC:Mammalia>
Protein name |
RecName:
|
Full=Cytosolic Fe-S cluster assembly factor NUBP1;
|
AltName:
|
Full=Nucleotide-binding protein 1;
|
|
Short=NBP 1;
|
|
else case <OC:Vertebrata>
Protein name |
RecName:
|
Full=Cytosolic Fe-S cluster assembly factor ;
|
AltName:
|
Full=Nucleotide-binding protein 1;
|
|
Short=NBP 1;
|
|
else case <OC:Fungi>
Protein name |
RecName:
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Full=Cytosolic Fe-S cluster assembly factor ;
|
AltName:
|
Full=Nucleotide-binding protein 35;
|
|
else case <OC:Viridiplantae>
Protein name |
RecName:
|
Full=Cytosolic Fe-S cluster assembly factor NBP35;
|
|
else
Protein name |
RecName:
|
Full=Cytosolic Fe-S cluster assembly factor NUBP1 homolog;
|
|
end case
case <OC:Vertebrata>
else case <OC:Fungi> or <OC:Viridiplantae>
end case
case <OC:Saccharomycetes>
Function |
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export. |
else case <OC:Fungi>
Function |
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. |
else case <OC:Viridiplantae>
Function |
Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development. |
else case <OC:Mammalia>
Function |
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. |
else
Function |
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. |
end case
case <OC:Viridiplantae>
Cofactor |
[4Fe-4S] cluster Note: Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable clusters in the N-termini and one labile, bridging cluster between subunits of the homodimer. |
else case <OC:Fungi>
Cofactor |
[4Fe-4S] cluster Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NBP35) and two labile, bridging clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer. |
else
Cofactor |
[4Fe-4S] cluster Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of @gn(NUBP1) and two labile, bridging clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer. |
end case
case <OC:Viridiplantae>
Subunit |
Homodimer and homotetramer. Predominantly homodimeric. |
else case <OC:Mammalia>
Subunit |
Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. |
else case <OC:Fungi>
Subunit |
Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains. |
else
Subunit |
Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains. |
end case
case <OC:Saccharomycetes>
Subcellular location |
Cytoplasm. Nucleus. |
else
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily. |
case <OC:Viridiplantae>
Miscellaneous |
Although plant and algal NBP35 proteins lack the characteristic CXXC motif in the C-terminus, thought to be required for Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C-terminus. Also, in this linage, no CFD1 partner protein homolog as found in other eukaryotes can be found. |
end case
case <FTGroup:1> or <FTGroup:2>
end case
case <FT:1>
end case
case <OC:Saccharomycetes>
end case
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0016226; Biological process: iron-sulfur cluster assembly.
GO:0005737; Cellular component: cytoplasm.
From: NBP35_YEAST (P52920) |
Key
|
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From
|
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To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
BINDING
|
|
80
|
|
87
|
|
/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616
|
|
|
|
G-K-G-G-V-G-K-S
|
|
|
BINDING
|
|
27
|
|
27
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1
|
|
|
|
C
|
|
1
|
BINDING
|
|
41
|
|
41
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1
|
|
|
|
C
|
|
1
|
BINDING
|
|
44
|
|
44
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1
|
|
|
|
C
|
|
1
|
BINDING
|
|
50
|
|
50
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1
|
|
|
|
C
|
|
1
|
case not <OC:Viridiplantae>
BINDING
|
|
253
|
|
253
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner
|
|
|
|
C
|
|
2
|
BINDING
|
|
256
|
|
256
|
|
/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="ligand shared with heterodimeric partner
|
|
|
|
C
|
|
2
|
end case
Additional information
[?]
Size range |
310-345 amino acids |
Related rules |
MF_02040 (APBC) |
Fusion |
None |