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Annotation rule MF_03053 |
General rule information
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Accession |
MF_03053 |
Dates |
26-FEB-2009 (Created) 20-NOV-2019 (Last updated, Version 4) |
Propagated annotation
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Identifier, protein and gene names
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Protein name |
RecName:
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Full=Cytoplasmic tRNA 2-thiolation protein 1;
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EC 2.7.7.-;
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AltName:
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Full=Cytoplasmic tRNA adenylyltransferase 1;
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case <OC:Vertebrata>
Protein name |
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Full=ATP-binding domain-containing protein 3;
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else case <OC:Caenorhabditis>
Protein name |
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Full=Thiolation of uridine in tRNA protein 1;
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else case <OC:Saccharomyces>
Protein name |
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Full=Thiolation of uridine in cytoplasmic tRNA protein 1;
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Full=Needs CLA4 to survive protein 6;
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else
Protein name |
RecName:
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Full=Cytoplasmic tRNA 2-thiolation protein 1;
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EC 2.7.7.-;
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AltName:
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Full=Cytoplasmic tRNA adenylyltransferase 1;
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end case
case <OC:Vertebrata>
else case <OC:Caenorhabditis>
else case <OC:Saccharomyces>
else case <OC:Fungi> or <OC:Viridiplantae>
end case
case <OC:Fungi>
Function |
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. |
else
Function |
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position. |
end case
Pathway |
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. |
case <OC:Mammalia>
Subunit |
Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2. |
else case <OC:Saccharomyces>
Subunit |
Interacts with NCS2 and URM1. May act by forming a heterodimer with NCS2. Component of a large molecular weight complex of more than 250 kDa. |
end case
case <OC:Saccharomyces>
Subcellular location |
Cytoplasm. Mitochondrion. |
else
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily. |
case <OC:Saccharomyces>
end case
GO:0000049; Molecular function: tRNA binding.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0032447; Biological process: protein urmylation.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
Additional information
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Size range |
313-443 amino acids |
Related rules |
None |
Fusion |
None |