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Annotation rule MF_03053
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General rule information [?]

Accession MF_03053
Dates 26-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 4)
Name CTU1
Scope
Eukaryota
Templates Q7Z7A3 (CTU1_HUMAN); P53088 (CTU1_YEAST); O94282 (CTU1_SCHPO): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CTU1
Protein name
RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1;
EC 2.7.7.-;
AltName: Full=Cytoplasmic tRNA adenylyltransferase 1;
case <OC:Vertebrata>
Protein name
Full=ATP-binding domain-containing protein 3;
else case <OC:Caenorhabditis>
Protein name
Full=Thiolation of uridine in tRNA protein 1;
else case <OC:Saccharomyces>
Protein name
Full=Thiolation of uridine in cytoplasmic tRNA protein 1;
Full=Needs CLA4 to survive protein 6;
else
Protein name
RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1;
EC 2.7.7.-;
AltName: Full=Cytoplasmic tRNA adenylyltransferase 1;
end case
case <OC:Vertebrata>
Gene name
CTU1, ATPBD3, NCS6
else case <OC:Caenorhabditis>
Gene name
tut-1, ctu-1
else case <OC:Saccharomyces>
Gene name
NCS6, CTU1, TUC1
else case <OC:Fungi> or <OC:Viridiplantae>
Gene name
NCS6, CTU1
end case

Comments [?]

case <OC:Fungi>
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
else
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position.
end case
Pathway tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
case <OC:Mammalia>
Subunit Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.
else case <OC:Saccharomyces>
Subunit Interacts with NCS2 and URM1. May act by forming a heterodimer with NCS2. Component of a large molecular weight complex of more than 250 kDa.
end case
case <OC:Saccharomyces>
Subcellular location Cytoplasm. Mitochondrion.
else
Subcellular location Cytoplasm.
end case
Similarity Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Keywords [?]

Cytoplasm
case <OC:Saccharomyces>
Mitochondrion
end case
RNA-binding
Transferase
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0000049; Molecular function: tRNA binding.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0002098; Biological process: tRNA wobble uridine modification.
GO:0032447; Biological process: protein urmylation.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01263; UPF0021; 1;
Pfam PF01171; ATP_bind_3; 1;
TIGRFAMs TIGR00269; UPF0021; 1;

Additional information [?]

Size range 313-443 amino acids
Related rules None
Fusion None


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