HAMAP rule MF_03100
General rule information
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| Accession | MF_03100 |
| Dates | 17-AUG-2009 (Created)
7-FEB-2023 (Last updated, Version 10) |
| Name | Endonuc_su_Slx1 |
| Scope(s) |
Eukaryota |
| Template(s) | P38324 (SLX1_YEAST); Q9BQ83 (SLX1_HUMAN); Q9P7M3 (SLX1_SCHPO); [ Recover all ] |
| Triggered by |
HAMAP; MF_03100 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SLX1 |
| case <OC:Vertebrata> | |
| Protein name | RecName: Full=Structure-specific endonuclease subunit EC=3.1.-.-; AltName: Full=GIY-YIG domain-containing protein 1; |
| else case <OC:Caenorhabditis> | |
| Protein name | RecName: Full=Structure-specific endonuclease subunit EC=3.1.-.-; AltName: Full=GIY-YIG domain-containing protein 1; |
| else case <OC:Fungi> | |
| Protein name | RecName: Full=Structure-specific endonuclease subunit EC=3.1.-.-; |
| else | |
| Protein name | RecName: Full=Structure-specific endonuclease subunit SLX1 homolog; EC=3.1.-.-; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=SLX1; Synonyms=GIYD1; |
| else case <OC:Caenorhabditis> | |
| Gene name | Name=slx-1; Synonyms=giyd-1; |
| else case <OC:Fungi> | |
| Gene name | Name=SLX1; |
| end case | |
Comments
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| case <OC:Mammalia> | |
| FUNCTION | Catalytic subunit of the @gn(SLX1)-@gn(SLX4) structure- specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. |
| else case <OC:Vertebrata> and not <OC:Mammalia> | |
| FUNCTION | Catalytic subunit of the @gn(SLX1)-@gn(SLX4) structure- specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
| else case <OC:Saccharomyces> | |
| FUNCTION | Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. |
| else case <OC:Fungi> and not <OC:Saccharomyces> | |
| FUNCTION | Catalytic subunit of the @gn(SLX1)-@gn(SLX4) structure- specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
| else | |
| FUNCTION | Catalytic subunit of a heterodimeric structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. |
| end case | |
| COFACTOR | Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; |
| case <OC:Vertebrata> or <OC:Fungi> | |
| SUBUNIT | Forms a heterodimer with @gn(SLX4). |
| else case <OC:Drosophilidae> | |
| SUBUNIT | Forms a heterodimer with mus312/SLX4. |
| else case <OC:Caenorhabditis> | |
| SUBUNIT | Forms a heterodimer with him-18/slx-4. |
| else | |
| SUBUNIT | Forms a heterodimer with a member of the SLX4 family. |
| end case | |
| SUBCELLULAR LOCATION | Nucleus. |
| SIMILARITY | Belongs to the SLX1 family. |
Keywords
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| DNA damage | |
| DNA recombination | |
| DNA repair | |
| Endonuclease | |
| Hydrolase | |
| Nuclease | |
| Nucleus | |
| case <FT:1> | |
| Metal-binding | |
| Zinc | |
| Zinc-finger | |
| end case | |
Gene Ontology
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| GO:0005634; Cellular component:nucleus | |
| GO:0033557; Cellular component:Slx1-Slx4 complex | |
| case <OCellular component:Mammalia> | |
| GO:0008821; Molecular function:crossover junction DNA endonuclease activity | |
| end case | |
| case <OCellular component:Mammalia> or <OC:Saccharomyces> | |
| GO:0017108; Molecular function:5'-flap endonuclease activity | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0004520; Molecular function:DNA endonuclease activity | |
| end case | |
| GO:0006310; Biological process:DNA recombination | |
| GO:0006281; Biological process:DNA repair | |
Cross-references
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| PROSITE | PS50164; GIY_YIG; 1; |
| PROSITE | PS01359; ZF_PHD_1; 0-1; |
| PROSITE | PS50016; ZF_PHD_2; 0-1; |
| Pfam | PF01541; GIY-YIG; 0-1; |
Features
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| From: SLX1_HUMAN (Q9BQ83) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ZN_FING | 186 | 238 | /note="SLX1-type" | C-x(1,2)-C-x*-C-x(1,7)-C-x(1,4)-[CH]-x(2)-C-x*-C-x(2)-C | ||||||||
Additional information
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| Size range | 246-705 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |