HAMAP rule MF_03137
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_03137 |
| Accession | MF_03137 |
| Dates | 2-NOV-2010 (Created)
3-SEP-2024 (Last updated, Version 12) |
| Name | GUF1 |
| Scope(s) |
Eukaryota |
| Template(s) | P46943 (GUF1_YEAST); Q8N442 (GUF1_HUMAN); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_00071 (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | GUF1 |
| case <OC:Vertebrata> or <OC:Fungi> | |
| Protein name | RecName: Full=Translation factor EC=3.6.5.n1; AltName: Full=Elongation factor 4 homolog; Short=EF-4; AltName: Full=GTPase AltName: Full=Ribosomal back-translocase; |
| else case <OC:Drosophilidae> | |
| Protein name | RecName: Full=Translation factor waclaw, mitochondrial; EC=3.6.5.n1; AltName: Full=Elongation factor 4 homolog; Short=EF-4; AltName: Full=GTPase GUF1 homolog; AltName: Full=Ribosomal back-translocase; |
| else | |
| Protein name | RecName: Full=Translation factor GUF1 homolog, mitochondrial; EC=3.6.5.n1; AltName: Full=Elongation factor 4 homolog; Short=EF-4; AltName: Full=GTPase GUF1 homolog; AltName: Full=Ribosomal back-translocase; |
| end case | |
| case <OC:Vertebrata> or <OC:Fungi> | |
| Gene name | Name=GUF1; |
| else case <OC:Drosophilidae> | |
| Gene name | Name=waw; |
| end case | |
Comments
[?]
| FUNCTION | Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner. |
| CATALYTIC ACTIVITY | Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1; |
| SUBCELLULAR LOCATION | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
| case not <AnyFeature:TransitM> | |
| MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
| end case | |
| SIMILARITY | Belongs to the GTP-binding elongation factor family. LepA subfamily. |
Keywords
[?]
| GTP-binding |
| Hydrolase |
| Membrane |
| Mitochondrion |
| Mitochondrion inner membrane |
| Nucleotide-binding |
| Protein biosynthesis |
Gene Ontology
[?]
| GO:0005759; Cellular component:mitochondrial matrix |
| GO:0005525; Molecular function:GTP binding |
| GO:0003924; Molecular function:GTPase activity |
| GO:0043022; Molecular function:ribosome binding |
| GO:0045727; Biological process:positive regulation of translation |
Cross-references
[?]
| Pfam | PF00679; EFG_C; 1; |
| Pfam | PF00009; GTP_EFTU; 1; |
| Pfam | PF03144; GTP_EFTU_D2; 1; |
| Pfam | PF06421; LepA_C; 1; |
| PRINTS | PR00315; ELONGATNFCT; 1; |
| NCBIfam | TIGR01393; lepA; 1; |
| NCBIfam | TIGR00231; Small_GTP; 1; |
| PROSITE | PS00301; G_TR_1; 1; |
| General | TransitM; -; 0-1; |
Features
[?]
| From: GUF1_YEAST (P46943) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 53 | 60 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[AS]-H-[IV]-D-[HS]-G-K-[ST] | ||||||||
| BINDING | 120 | 124 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-[CP]-G-H | ||||||||
| BINDING | 174 | 177 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[NT]-K-[IMV]-D | ||||||||
Additional information
[?]
| Size range | 627-1085 amino acids |
| Related rules |
MF_03138 |
| Fusion | Nter: None Cter: None |