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HAMAP rule MF_03137

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General rule information [?]

Accession MF_03137
Dates 2-NOV-2010 (Created)
1-JUN-2023 (Last updated, Version 11)
Name GUF1
Scope(s) Eukaryota
Template(s) P46943 (GUF1_YEAST); Q8N442 (GUF1_HUMAN); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_00071 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GUF1
case <OC:Vertebrata> or <OC:Fungi>
RecName: Full=Translation factor <gene_name>, mitochondrial;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Short=EF-4;<br /> AltName: Full=GTPase <gene_name>;<br /> AltName: Full=Ribosomal back-translocase;
else case <OC:Drosophilidae>
RecName: Full=Translation factor waclaw, mitochondrial;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase;
else
RecName: Full=Translation factor GUF1 homolog, mitochondrial;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase;
end case
case <OC:Vertebrata> or <OC:Fungi>
Gene name Name=GUF1;
else case <OC:Drosophilidae>
Gene name Name=waw;
end case

Comments [?]

FUNCTIONPromotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
CATALYTIC ACTIVITY Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
SUBCELLULAR LOCATIONMitochondrion inner membrane; Peripheral membrane protein; Matrix side.
case not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the GTP-binding elongation factor family. LepA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005759; Cellular component:mitochondrial matrix
GO:0005525; Molecular function:GTP binding
GO:0003924; Molecular function:GTPase activity
GO:0043022; Molecular function:ribosome binding
GO:0045727; Biological process:positive regulation of translation

Cross-references [?]

Pfam PF00679; EFG_C; 1;
Pfam PF00009; GTP_EFTU; 1;
Pfam PF03144; GTP_EFTU_D2; 1;
Pfam PF06421; LepA_C; 1;
PRINTS PR00315; ELONGATNFCT; 1;
NCBIfam TIGR01393; lepA; 1;
NCBIfam TIGR00231; Small_GTP; 1;
PROSITE PS00301; G_TR_1; 1;
General TransitM; -; 0-1;

Features [?]

From: GUF1_YEAST (P46943)
Key From To Description Tag Condition FTGroup
BINDING 53 60 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
[AS]-H-[IV]-D-[HS]-G-K-[ST]
BINDING 120 124 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
D-T-[CP]-G-H
BINDING 174 177 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
[NT]-K-[IMV]-D

Additional information [?]

Size range 627-1085 amino acids
Related rules MF_03138
Fusion Nter: None Cter: None



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