HAMAP rule MF_03137
General rule information
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Accession | MF_03137 |
Dates | 2-NOV-2010 (Created)
1-JUN-2023 (Last updated, Version 11) |
Name | GUF1 |
Scope(s) |
Eukaryota |
Template(s) | P46943 (GUF1_YEAST); Q8N442 (GUF1_HUMAN); [ Recover all ] |
Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_00071 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | GUF1 |
case <OC:Vertebrata> or <OC:Fungi> | |
RecName: Full=Translation factor <gene_name>, mitochondrial;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> Short=EF-4;<br /> AltName: Full=GTPase <gene_name>;<br /> AltName: Full=Ribosomal back-translocase; | |
else case <OC:Drosophilidae> | |
RecName: Full=Translation factor waclaw, mitochondrial;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase; | |
else | |
RecName: Full=Translation factor GUF1 homolog, mitochondrial;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br /> Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase; | |
end case | |
case <OC:Vertebrata> or <OC:Fungi> | |
Gene name | Name=GUF1; |
else case <OC:Drosophilidae> | |
Gene name | Name=waw; |
end case |
Comments
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FUNCTION | Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner. |
CATALYTIC ACTIVITY | Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1; |
SUBCELLULAR LOCATION | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
case not <AnyFeature:TransitM> | |
MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case | |
SIMILARITY | Belongs to the GTP-binding elongation factor family. LepA subfamily. |
Keywords
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GTP-binding |
Hydrolase |
Membrane |
Mitochondrion |
Mitochondrion inner membrane |
Nucleotide-binding |
Protein biosynthesis |
Gene Ontology
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GO:0005759; Cellular component:mitochondrial matrix |
GO:0005525; Molecular function:GTP binding |
GO:0003924; Molecular function:GTPase activity |
GO:0043022; Molecular function:ribosome binding |
GO:0045727; Biological process:positive regulation of translation |
Cross-references
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Pfam | PF00679; EFG_C; 1; |
Pfam | PF00009; GTP_EFTU; 1; |
Pfam | PF03144; GTP_EFTU_D2; 1; |
Pfam | PF06421; LepA_C; 1; |
PRINTS | PR00315; ELONGATNFCT; 1; |
NCBIfam | TIGR01393; lepA; 1; |
NCBIfam | TIGR00231; Small_GTP; 1; |
PROSITE | PS00301; G_TR_1; 1; |
General | TransitM; -; 0-1; |
Features
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From: GUF1_YEAST (P46943) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 53 | 60 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[AS]-H-[IV]-D-[HS]-G-K-[ST] | ||||||||
BINDING | 120 | 124 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-[CP]-G-H | ||||||||
BINDING | 174 | 177 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[NT]-K-[IMV]-D |
Additional information
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Size range | 627-1085 amino acids |
Related rules |
MF_03138 |
Fusion | Nter: None Cter: None |