HAMAP rule MF_03137

General rule information [?]

Accession	MF_03137
Dates	2-NOV-2010 (Created) 1-JUN-2023 (Last updated, Version 11)
Name	GUF1
Scope(s)	Eukaryota
Template(s)	P46943 (GUF1_YEAST); Q8N442 (GUF1_HUMAN); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_00071 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	GUF1
case <OC:Vertebrata> or <OC:Fungi>
RecName: Full=Translation factor <gene_name>, mitochondrial;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br />                  Short=EF-4;<br /> AltName: Full=GTPase <gene_name>;<br /> AltName: Full=Ribosomal back-translocase;
else case <OC:Drosophilidae>
RecName: Full=Translation factor waclaw, mitochondrial;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br />                  Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase;
else
RecName: Full=Translation factor GUF1 homolog, mitochondrial;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Elongation factor 4 homolog;<br />                  Short=EF-4;<br /> AltName: Full=GTPase GUF1 homolog;<br /> AltName: Full=Ribosomal back-translocase;
end case
case <OC:Vertebrata> or <OC:Fungi>
Gene name	Name=GUF1;
else case <OC:Drosophilidae>
Gene name	Name=waw;
end case

Comments [?]

FUNCTION	Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
CATALYTIC ACTIVITY	Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
SUBCELLULAR LOCATION	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
case not <AnyFeature:TransitM>
MISCELLANEOUS	This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITY	Belongs to the GTP-binding elongation factor family. LepA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005759; Cellular component:mitochondrial matrix

GO:0005525; Molecular function:GTP binding

GO:0003924; Molecular function:GTPase activity

GO:0043022; Molecular function:ribosome binding

GO:0045727; Biological process:positive regulation of translation

Cross-references [?]

Features [?]

From: GUF1_YEAST (P46943)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

[AS]-H-[IV]-D-[HS]-G-K-[ST]

BINDING

120

124

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

D-T-[CP]-G-H

BINDING

174

177

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

[NT]-K-[IMV]-D

Additional information [?]