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Annotation rule MF_03143
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General rule information [?]

Accession MF_03143
Dates 16-FEB-2011 (Created)
20-NOV-2019 (Last updated, Version 8)
Name Pentafunct_AroM
Scope
Eukaryota; Fungi
Templates P07547 (ARO1_EMENI); P08566 (ARO1_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ARO1
Protein name
RecName: Full=Pentafunctional AROM polypeptide;
Includes:
RecName: Full=3-dehydroquinate synthase;
Short=DHQS;
EC 4.2.3.4;
Includes:
RecName: Full=3-phosphoshikimate 1-carboxyvinyltransferase;
EC 2.5.1.19;
AltName: Full=5-enolpyruvylshikimate-3-phosphate synthase;
Short=EPSP synthase;
Short=EPSPS;
Includes:
RecName: Full=Shikimate kinase;
Short=SK;
EC 2.7.1.71;
Includes:
RecName: Full=3-dehydroquinate dehydratase;
Short=3-dehydroquinase;
EC 4.2.1.10;
Includes:
RecName: Full=Shikimate dehydrogenase;
EC 1.1.1.25;
case <OC:Trichocomaceae>
Gene name
aroM
else case <OC:Saccharomycetales>
Gene name
ARO1
else case <OC:Schizosaccharomyces>
Gene name
aro1
else case <OC:Neurospora>
Gene name
aro-1
end case

Comments [?]

Function The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.
Catalytic activity RHEA:21968: 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate
EC 4.2.3.4
RHEA:21096: 3-dehydroquinate = 3-dehydroshikimate + H2O
EC 4.2.1.10
RHEA:17737: NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH
EC 1.1.1.25
RHEA:13121: ATP + shikimate = 3-phosphoshikimate + ADP + H(+)
EC 2.7.1.71
RHEA:21256: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate
EC 2.5.1.19
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit.
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.
In the 2nd section; belongs to the EPSP synthase family.
In the 3rd section; belongs to the shikimate kinase family.
In the 4th section; belongs to the type-I 3-dehydroquinase family.
In the C-terminal section; belongs to the shikimate dehydrogenase family.

Keywords [?]


Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0003855; Molecular function: 3-dehydroquinate dehydratase activity.
GO:0003856; Molecular function: 3-dehydroquinate synthase activity.
GO:0003866; Molecular function: 3-phosphoshikimate 1-carboxyvinyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0046872; Molecular function: metal ion binding.
GO:0004764; Molecular function: shikimate 3-dehydrogenase (NADP+) activity.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0055114; Biological process: oxidation-reduction process.

Cross-references [?]

PROSITE PS01028; DEHYDROQUINASE_I; 1;
PS00104; EPSP_SYNTHASE_1; 1;
PS00885; EPSP_SYNTHASE_2; 1;
PS01128; SHIKIMATE_KINASE; 1;
Pfam PF01487; DHquinase_I; 1;
PF00275; EPSP_synthase; 1;
PF01488; Shikimate_DH; 1;
PF08501; Shikimate_dh_N; 1;
PF01202; SKI; 1;
PF01761; DHQ_synthase; 1;
PRINTS PR01100; SHIKIMTKNASE; 1;
TIGRFAMs TIGR01356; aroA; 1;
TIGR01357; aroB; 1;
TIGR01093; aroD; 1;
TIGR01809; Shik-DH-AROM; 1;
PIRSF PIRSF001455; DHQ_synth; 1;
PIRSF000514; Pentafunct_AroM; 1;

Features [?]

From: ARO1_EMENI (P07547)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     44     46       NAD     D-[TSDQ]-[NRT]  
NP_BIND     81     84       NAD     E-x(2)-K  
NP_BIND     114     116       NAD     G-G-[VI]  
NP_BIND     139     140       NAD     T-[TS]  
NP_BIND     179     182       NAD     [FYW]-[LI]-x-[TS]  
NP_BIND     870     877       ATP     G-M-R-[GA]-[AST]-G-K-[ST]  
REGION     Nter     384       3-dehydroquinate synthase        
REGION     194     197       Substrate binding 2     E-[VIL]-[IV]-K  
REGION     264     268       Substrate binding 2     R-[NT]-[LI]-[LV]-N  
REGION     397     842       EPSP synthase        
REGION     863     1055       Shikimate kinase        
REGION     1056     1276       3-dehydroquinase        
REGION     1289     Cter       Shikimate dehydrogenase        
ACT_SITE     260     260       Proton acceptor; for 3-dehydroquinate synthase activity     E  
ACT_SITE     275     275       Proton acceptor; for 3-dehydroquinate synthase activity     H  
ACT_SITE     824     824       For EPSP synthase activity     C  
ACT_SITE     1179     1179       Proton acceptor; for 3-dehydroquinate dehydratase activity     H  
ACT_SITE     1207     1207       Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity     [KR]  
METAL     194     194       Zinc; catalytic     E  
METAL     271     271       Zinc; catalytic     H  
METAL     287     287       Zinc; catalytic     H  
BINDING     119     119       NAD     D  
BINDING     130     130       Substrate 1     R  
BINDING     146     146       Substrate 2     D  
BINDING     152     152       Substrate 2     K  
BINDING     161     161       NAD     K  
BINDING     162     162       Substrate 2     N  
BINDING     190     190       NAD     N  
BINDING     250     250       Substrate 2     K  
BINDING     271     271       Substrate 2     H  
BINDING     287     287       Substrate 2     H  
BINDING     356     356       Substrate 2     K  

Additional information [?]

Size range 1523-1715 amino acids
Related rules MF_00109 (AROK); MF_00110 (AROB); MF_00210 (AROA); MF_00214 (AROD); MF_00222 (AROE)
Fusion None