HAMAP rule MF_03146
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_03146 |
| Accession | MF_03146 |
| Dates | 13-MAY-2011 (Created)
3-SEP-2024 (Last updated, Version 12) |
| Name | TSR3_euk |
| Scope(s) |
Eukaryota |
| Template(s) | Q12094 (TSR3_YEAST); Q9UJK0 (TSR3_HUMAN); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_01116 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TSR3 |
| Protein name | RecName: Full=18S rRNA aminocarboxypropyltransferase; EC=2.5.1.157; |
| case <OC:Fungi> | |
| Gene name | Name=TSR3; |
| end case | |
Comments
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| case <OC:Vertebrata> | |
| FUNCTION | Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1248 (Psi1248) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. |
| CATALYTIC ACTIVITY | Reaction=an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+); Xref=Rhea:RHEA:63296, Rhea:RHEA- COMP:11634, Rhea:RHEA-COMP:16310, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; EC=2.5.1.157; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63297; |
| CATALYTIC ACTIVITY | Reaction=N(1)-methylpseudouridine(1248) in human 18S rRNA + S-adenosyl- L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3- carboxypropyl]pseudouridine(1248) in human 18S rRNA + S-methyl-5'- thioadenosine + H(+); Xref=Rhea:RHEA:63292, Rhea:RHEA-COMP:11639, Rhea:RHEA- COMP:16308, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63293; |
| else case <OC:Fungi> | |
| FUNCTION | Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. |
| CATALYTIC ACTIVITY | Reaction=an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+); Xref=Rhea:RHEA:63296, Rhea:RHEA- COMP:11634, Rhea:RHEA-COMP:16310, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; EC=2.5.1.157; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63297; |
| CATALYTIC ACTIVITY | Reaction=N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl- L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3- carboxypropyl]pseudouridine(1191) in yeast 18S rRNA + S-methyl-5'- thioadenosine + H(+); Xref=Rhea:RHEA:63300, Rhea:RHEA-COMP:13852, Rhea:RHEA- COMP:16309, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63301; |
| else | |
| FUNCTION | Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1- methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi). |
| CATALYTIC ACTIVITY | Reaction=an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+); Xref=Rhea:RHEA:63296, Rhea:RHEA- COMP:11634, Rhea:RHEA-COMP:16310, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; EC=2.5.1.157; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63297; |
| end case | |
| case <OC:Vertebrata> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| else case <OC:Fungi> | |
| SUBCELLULAR LOCATION | Cytoplasm. Nucleus. |
| end case | |
| SIMILARITY | Belongs to the TDD superfamily. TSR3 family. |
Keywords
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| case <OC:Vertebrata> or <OC:Fungi> | |
| Cytoplasm | |
| end case | |
| case <OC:Fungi> | |
| Nucleus | |
| end case | |
| Ribosome biogenesis | |
| S-adenosyl-L-methionine | |
| Transferase | |
| rRNA processing | |
Gene Ontology
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| case <OCellular component:Vertebrata> or <OC:Fungi> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
| case <OCellular component:Fungi> | |
| GO:0005634; Cellular component:nucleus | |
| end case | |
| GO:1904047; Molecular function:S-adenosyl-L-methionine binding | |
| GO:0006364; Biological process:rRNA processing | |
| GO:0000455; Biological process:enzyme-directed rRNA pseudouridine synthesis | |
Cross-references
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Features
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| From: TSR3_YEAST (Q12094) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 62 | 62 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ST] | ||||||||
| BINDING | 110 | 110 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[VIL] | ||||||||
| BINDING | 133 | 133 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LI] | ||||||||
| BINDING | 148 | 148 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[WY] | ||||||||
Additional information
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| Size range | 247-421 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |