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HAMAP rule MF_03146

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General rule information [?]

Accession MF_03146
Dates 13-MAY-2011 (Created)
19-NOV-2022 (Last updated, Version 10)
Name TSR3_euk
Scope(s) Eukaryota
Template(s) Q12094 (TSR3_YEAST); Q9UJK0 (TSR3_HUMAN); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01116 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TSR3
Protein name RecName: Full=18S rRNA aminocarboxypropyltransferase;
                 EC=2.5.1.-;
case <OC:Fungi>
Gene name Name=TSR3;
end case

Comments [?]

case <OC:Vertebrata>
FUNCTIONAminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1248 (Psi1248) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.
CATALYTIC ACTIVITY Reaction=N(1)-methylpseudouridine(1248) in human 18S rRNA + S-adenosyl- L-methionine = H(+) + N(1)-methyl-N(3)-[(3S)-3-amino-3- carboxypropyl]pseudouridine(1248) in human 18S rRNA + S-methyl-5'- thioadenosine; Xref=Rhea:RHEA:63292, Rhea:RHEA-COMP:11639, Rhea:RHEA- COMP:16308, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63293;
else case <OC:Fungi>
FUNCTIONAminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.
CATALYTIC ACTIVITY Reaction=N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl- L-methionine = H(+) + N(1)-methyl-N(3)-[(3S)-3-amino-3- carboxypropyl]pseudouridine(1191) in yeast 18S rRNA + S-methyl-5'- thioadenosine; Xref=Rhea:RHEA:63300, Rhea:RHEA-COMP:13852, Rhea:RHEA- COMP:16309, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63301;
else
FUNCTIONAminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1- methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).
CATALYTIC ACTIVITY Reaction=an N(1)-methylpseudouridine in 16S/18S rRNA + S-adenosyl-L- methionine = H(+) + N(1)-methyl-N(3)[(3S)-3-amino-3- carboxypropyl]pseudouridine in 16S/18S rRNA + S-methyl-5'- thioadenosine; Xref=Rhea:RHEA:63296, Rhea:RHEA-COMP:11634, Rhea:RHEA- COMP:16310, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:74890, ChEBI:CHEBI:146234; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63297;
end case
case <OC:Vertebrata>
SUBCELLULAR LOCATIONCytoplasm.
else case <OC:Fungi>
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
end case
SIMILARITYBelongs to the TDD superfamily. TSR3 family.

Keywords [?]

case <OC:Vertebrata> or <OC:Fungi>
Cytoplasm
end case
case <OC:Fungi>
Nucleus
end case
Ribosome biogenesis
S-adenosyl-L-methionine
Transferase
rRNA processing

Gene Ontology [?]

case <OCellular component:Vertebrata> or <OC:Fungi>
GO:0005737; Cellular component:cytoplasm
end case
case <OCellular component:Fungi>
GO:0005634; Cellular component:nucleus
end case
GO:1904047; Molecular function:S-adenosyl-L-methionine binding
GO:0006364; Biological process:rRNA processing
GO:0000455; Biological process:enzyme-directed rRNA pseudouridine synthesis

Cross-references [?]

Pfam PF04034; Ribo_biogen_C; 1;
Pfam PF04068; RLI; 1;

Features [?]

From: TSR3_YEAST (Q12094)
Key From To Description Tag Condition FTGroup
BINDING 62 62 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[ST]
BINDING 110 110 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[VIL]
BINDING 133 133 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[LI]
BINDING 148 148 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[WY]

Additional information [?]

Size range 247-421 amino acids
Related rules None
Fusion Nter: None Cter: None



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