HAMAP rule MF_03150

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_03150
Accession	MF_03150
Dates	22-AUG-2012 (Created) 22-MAY-2026 (Last updated, Version )
Name	GatA_euk
Scope(s)	Eukaryota
Template(s)	Q9H0R6 (GATA_HUMAN); Q9LI77 (GATA_ARATH); Q03557 (GATA_YEAST); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_00120 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	GATA
case <OC:Viridiplantae>
Protein name	RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial; Short=Glu-AdT subunit A; EC=3.5.1.2;
else case <OC:Vertebrata>
Protein name	RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial; Short=Glu-AdT subunit A; EC=3.5.1.2; AltName: Full=Glutaminyl-tRNA synthase-like protein 1;
else
Protein name	RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial; Short=Glu-AdT subunit A; EC=3.5.1.2;
end case
case <OC:Vertebrata>
Gene name	Name=QRSL1;
else case <OC:Saccharomycotina>
Gene name	Name=HER2;
else case <OC:Viridiplantae>
Gene name	Name=GATA;
else case <OC:Insecta>
Gene name	Name=GatA;
end case

Comments [?]

case <OC:Viridiplantae>
FUNCTION	Catalytic subunit of the mitochondrial Glu-tRNA(Gln) amidotransferase complex GatCAB. GatCAB allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This subunit probably catalyzes the hydrolysis of the amido donor Gln, releasing ammonia that is sequestered within a channel in the GatCAB complex and made available to subunit B for downstream aminolysis of Glu-tRNA(Gln).
else
FUNCTION	Catalytic subunit of the mitochondrial Glu-tRNA(Gln) amidotransferase complex GatCAB. GatCAB allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma- phospho-Glu-tRNA(Gln). This subunit probably catalyzes the hydrolysis of the amido donor Gln, releasing ammonia that is sequestered within a channel in the GatCAB complex and made available to subunit B for downstream aminolysis of Glu-tRNA(Gln).
end case
CATALYTIC ACTIVITY	Reaction=L-glutamine + H2O = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
case <OC:Vertebrata>
SUBUNIT	Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (@gn(QRSL1)), B (@gn(GATB)) and C (@gn(GATC)) subunits.
else case <OC:Saccharomycotina>
SUBUNIT	Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.
else
SUBUNIT	Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
end case
case <OC:Viridiplantae>
SUBCELLULAR LOCATION	Mitochondrion. Plastid, chloroplast stroma.
else
SUBCELLULAR LOCATION	Mitochondrion.
end case
case <OC:Viridiplantae>
MISCELLANEOUS	This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITY	Belongs to the amidase family. GatA subfamily.

Keywords [?]

case <OC:Viridiplantae>
Chloroplast
Plastid
end case
Mitochondrion
Hydrolase
Protein biosynthesis

Gene Ontology [?]

case <OCellular component:Viridiplantae>
GO:0009507; Cellular component:chloroplast
end case
GO:0005739; Cellular component:mitochondrion
GO:0030956; Cellular component:glutamyl-tRNA(Gln) amidotransferase complex
GO:0004359; Molecular function:glutaminase activity
GO:0070681; Biological process:glutaminyl-tRNAGln biosynthesis via transamidation
GO:0032543; Biological process:mitochondrial translation

Cross-references [?]

PROSITE	PS00571; AMIDASES; 1;
Pfam	PF01425; Amidase; 1;
NCBIfam	TIGR00132; GatA; 1;

Features [?]

From: GATA_HUMAN (Q9H0R6)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

/note="Charge relay system"

ACT_SITE

171

/note="Charge relay system"

ACT_SITE

195

/note="Acyl-ester intermediate"

From: GATA_YEAST (Q03557)

Key

From

Description

Tag

Condition

FTGroup

BINDING

103