HAMAP rule MF_03159
General rule information
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Accession | MF_03159 |
Dates | 14-FEB-2012 (Created) 1-JUN-2023 (Last updated, Version 15) |
Name | NADHX_epimerase_euk |
Scope | Eukaryota |
Templates | Q9X024 (NNR_THEMA); Q8NCW5 (NNRE_HUMAN): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Vertebrata>
Protein name |
|
Gene name |
|
else
Protein name |
|
end case
Comments
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case <OC:Mammalia>
Function | Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis. |
else
Function | Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. |
end case
Catalytic activity | RHEA:32215: (6R)-NADHX = (6S)-NADHX
EC 5.1.99.6 |
RHEA:32227: (6R)-NADPHX = (6S)-NADPHX
EC 5.1.99.6 |
|
Cofactor | K(+) Note: Binds 1 potassium ion per subunit. |
case <OC:Fungi>
Subcellular location | Cytoplasm. Mitochondrion. |
else case <OC:Mammalia>
Subunit | Homodimer. Interacts with @gn(APOA1) and @gn(APOA2). |
Subcellular location | Mitochondrion. Secreted. Note=In sperm, secretion gradually increases during capacitation. |
Ptm | Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation. |
else case <OC:Vertebrata> and not <OC:Mammalia>
Subcellular location | Mitochondrion. Secreted. |
end case
case <OC:Vertebrata> and not <AnyFeature:TransitM>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity | Belongs to the NnrE/AIBP family. |
Keywords
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case <OC:Fungi>
else case <OC:Vertebrata>
end case
Gene Ontology
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GO:0052856; Molecular function: NADHX epimerase activity.
case <OC:Fungi>
GO:0005737; Cellular component: cytoplasm.
else case <OC:Vertebrata>
GO:0005739; Cellular component: mitochondrion.
end case
Cross-references
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PROSITE | PS51385; YJEF_N; 1; trigger=PRU00719; |
Pfam | PF03853; YjeF_N; 1; |
NCBIfam | TIGR00197; YjeF_nterm; 1; |
Computed features
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case <OC:Vertebrata>
General | TransitM; -; 0-1; trigger=yes; |
end case
Features
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From: NNR_THEMA (Q9X024) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 52 | 52 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 | [NQ] | ||||||||
BINDING | 114 | 114 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 | D | ||||||||
BINDING | 150 | 150 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 | [ST] | ||||||||
BINDING | 51 | 55 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | x-[NQ]-G-x(2) | ||||||||
BINDING | 118 | 124 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | |||||||||
BINDING (Optional) | 129 | 129 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | Y | ||||||||
BINDING | 147 | 147 | /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076 | [DE] |
Additional information
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Size range | 204-292 amino acids |
Related rules | None |
Fusion | None |