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HAMAP rule MF_03159

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General rule information [?]

Accession MF_03159
Dates 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 15)
Name NADHX_epimerase_euk
Scope(s) Eukaryota
Template(s) Q9X024 (NNR_THEMA); Q8NCW5 (NNRE_HUMAN); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01966 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NNRE
case <OC:Vertebrata>
Protein name RecName: Full=NAD(P)H-hydrate epimerase;
                 EC=5.1.99.6;
AltName: Full=Apolipoprotein A-I-binding protein;
                 Short=AI-BP;
AltName: Full=NAD(P)HX epimerase;
Gene name Name=APOA1BP; Synonyms=AIBP;
else
Protein name RecName: Full=NAD(P)H-hydrate epimerase;
                 EC=5.1.99.6;
AltName: Full=NAD(P)HX epimerase;
end case

Comments [?]

case <OC:Mammalia>
FUNCTIONCatalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis.
else
FUNCTIONCatalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
end case
CATALYTIC ACTIVITY Reaction=(6R)-NADHX = (6S)-NADHX; Xref=Rhea:RHEA:32215, ChEBI:CHEBI:64074, ChEBI:CHEBI:64075; EC=5.1.99.6;
CATALYTIC ACTIVITY Reaction=(6R)-NADPHX = (6S)-NADPHX; Xref=Rhea:RHEA:32227, ChEBI:CHEBI:64076, ChEBI:CHEBI:64077; EC=5.1.99.6;
COFACTOR Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Binds 1 potassium ion per subunit.;
case <OC:Fungi>
SUBCELLULAR LOCATIONCytoplasm. Mitochondrion.
else case <OC:Mammalia>
SUBUNITHomodimer. Interacts with @gn(APOA1) and @gn(APOA2).
SUBCELLULAR LOCATIONMitochondrion. Secreted. Note=In sperm, secretion gradually increases during capacitation.
PTMUndergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.
else case <OC:Vertebrata> and not <OC:Mammalia>
SUBCELLULAR LOCATIONMitochondrion. Secreted.
end case
case <OC:Vertebrata> and not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the NnrE/AIBP family.

Keywords [?]

Isomerase
Metal-binding
NAD
Nucleotide-binding
Potassium
case <OC:Fungi>
Cytoplasm
Mitochondrion
else case <OC:Vertebrata>
Mitochondrion
Secreted
end case

Gene Ontology [?]

GO:0052856; Molecular function:NADHX epimerase activity
case <OCellular component:Fungi>
GO:0005737; Cellular component:cytoplasm
else case <OCellular component:Vertebrata>
GO:0005739; Cellular component:mitochondrion
end case

Cross-references [?]

PROSITE PS51385; YJEF_N; 1;
Pfam PF03853; YjeF_N; 1;
NCBIfam TIGR00197; YjeF_nterm; 1;
General TransitM; -; 0-1;

Features [?]

From: NNR_THEMA (Q9X024)
Key From To Description Tag Condition FTGroup
BINDING 52 52 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"		 [NQ]
BINDING 114 114 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"		 D
BINDING 150 150 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"		 [ST]
BINDING 51 55 /ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"		 x-[NQ]-G-x(2)
BINDING 118 124 /ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"
BINDING 129 129 /ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"		 Y
BINDING 147 147 /ligand="(6S)-NADPHX"
/ligand_id="ChEBI:CHEBI:64076"		 [DE]

Additional information [?]

Size range 204-292 amino acids
Related rules None
Fusion Nter: None Cter: None



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