HAMAP logo

HAMAP rule MF_03159

Send feedback

General rule information [?]

Accession MF_03159
Dates 14-FEB-2012 (Created)
1-JUN-2023 (Last updated, Version 15)
Name NADHX_epimerase_euk
Scope
Eukaryota
Templates Q9X024 (NNR_THEMA); Q8NCW5 (NNRE_HUMAN): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NNRE
case <OC:Vertebrata>
Protein name
RecName: Full=NAD(P)H-hydrate epimerase;
EC 5.1.99.6;
AltName: Full=Apolipoprotein A-I-binding protein;
Short=AI-BP;
AltName: Full=NAD(P)HX epimerase;
Gene name
APOA1BP, AIBP
else
Protein name
RecName: Full=NAD(P)H-hydrate epimerase;
EC 5.1.99.6;
AltName: Full=NAD(P)HX epimerase;
end case

Comments [?]

case <OC:Mammalia>
Function Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis.
else
Function Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
end case
Catalytic activity RHEA:32215: (6R)-NADHX = (6S)-NADHX
EC 5.1.99.6
RHEA:32227: (6R)-NADPHX = (6S)-NADPHX
EC 5.1.99.6
Cofactor K(+)
Note: Binds 1 potassium ion per subunit.
case <OC:Fungi>
Subcellular location Cytoplasm. Mitochondrion.
else case <OC:Mammalia>
Subunit Homodimer. Interacts with @gn(APOA1) and @gn(APOA2).
Subcellular location Mitochondrion. Secreted. Note=In sperm, secretion gradually increases during capacitation.
Ptm Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.
else case <OC:Vertebrata> and not <OC:Mammalia>
Subcellular location Mitochondrion. Secreted.
end case
case <OC:Vertebrata> and not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the NnrE/AIBP family.

Keywords [?]

Isomerase
Metal-binding
NAD
Nucleotide-binding
Potassium
case <OC:Fungi>
Cytoplasm
Mitochondrion
else case <OC:Vertebrata>
Mitochondrion
Secreted
end case

Gene Ontology [?]

GO:0052856; Molecular function: NADHX epimerase activity.
case <OC:Fungi>
GO:0005737; Cellular component: cytoplasm.
else case <OC:Vertebrata>
GO:0005739; Cellular component: mitochondrion.
end case

Cross-references [?]

PROSITE PS51385; YJEF_N; 1; trigger=PRU00719;
Pfam PF03853; YjeF_N; 1;
NCBIfam TIGR00197; YjeF_nterm; 1;

Computed features [?]

case <OC:Vertebrata>
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

From: NNR_THEMA (Q9X024)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     52     52       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     [NQ]  
BINDING     114     114       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     D  
BINDING     150     150       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     [ST]  
BINDING     51     55       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076     x-[NQ]-G-x(2)  
BINDING     118     124       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076        
BINDING (Optional)     129     129       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076     Y  
BINDING     147     147       /ligand="(6S)-NADPHX" /ligand_id="ChEBI:CHEBI:64076     [DE]  

Additional information [?]

Size range 204-292 amino acids
Related rules None
Fusion None


View rule in raw text format (no links)