HAMAP rule MF

Accession	MF_03176
Dates	31-JUL-2013 (Created) 19-NOV-2022 (Last updated, Version 10)

Name

PIF1

Scope

Eukaryota

Templates

P07271 (PIF1_YEAST); Q9H611 (PIF1_HUMAN); Q9UUA2 (PIF1_SCHPO): [Recover all]

Triggered by

HAMAP; MF_03176 (Get profile general information and statistics)

Identifier

PIF1

Protein name

RecName:		Full=ATP-dependent DNA helicase PIF1;
		EC 3.6.4.12;
AltName:		Full=DNA repair and recombination helicase PIF1;

Gene name

PIF1

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability.

Catalytic activity	RHEA:13065: ATP + H2O = ADP + H(+) + phosphate EC 3.6.4.12
Cofactor	Mg(2+)

Subunit

Monomer. Interacts with telomerase.

Subunit

Monomer.

Subcellular location	Nucleus. Mitochondrion.
Similarity	Belongs to the helicase family. PIF1 subfamily.

ATP-binding
DNA damage
DNA recombination
DNA repair
DNA-binding
Helicase
Hydrolase
Mitochondrion
Nucleotide-binding
Nucleus

GO:0051880; Molecular function: G-quadruplex DNA binding.
GO:0010521; Molecular function: telomerase inhibitor activity.
GO:0032211; Biological process: negative regulation of telomere maintenance via telomerase.

GO:0005739; Cellular component: mitochondrion.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0043139; Molecular function: 5'-3' DNA helicase activity.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0000002; Biological process: mitochondrial genome maintenance.

Pfam	PF05970; PIF1; 1-2;

From: PIF1_YEAST (P07271)

Key From To Description Tag Condition FTGroup

BINDING 258 265 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-x-A-G-T-G-K-S

DNA_BIND 727 746 [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F

Size range	593-1113 amino acids
Related rules	MF_03177 (RRM3 supersedes the current rule)
Fusion	None

View rule in raw text format (no links)

HAMAP rule MF_03176