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Annotation rule MF_03176
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General rule information [?]

Accession MF_03176
Dates 31-JUL-2013 (Created)
20-NOV-2019 (Last updated, Version 8)
Name PIF1
Scope
Eukaryota
Templates P07271 (PIF1_YEAST); Q9H611 (PIF1_HUMAN); Q9UUA2 (PIF1_SCHPO): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PIF1
Protein name
RecName: Full=ATP-dependent DNA helicase PIF1;
EC 3.6.4.12;
AltName: Full=DNA repair and recombination helicase PIF1;
Gene name
PIF1

Comments [?]

case <OC:Saccharomycetales>
Function DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.
else case <OC:Mammalia>
Function DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.
else
Function DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability.
end case
Catalytic activity RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
EC 3.6.4.12
Cofactor Mg(2+)
case <OC:Saccharomycetales> or <OC:Mammalia>
Subunit Monomer. Interacts with telomerase.
else
Subunit Monomer.
end case
Subcellular location Nucleus. Mitochondrion.
Similarity Belongs to the helicase family. PIF1 subfamily.

Keywords [?]


Gene Ontology [?]

case <OC:Saccharomycetales> or <OC:Mammalia>
GO:0051880; Molecular function: G-quadruplex DNA binding.
GO:0010521; Molecular function: telomerase inhibitor activity.
GO:0032211; Biological process: negative regulation of telomere maintenance via telomerase.
end case
GO:0005739; Cellular component: mitochondrion.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0043139; Molecular function: 5'-3' DNA helicase activity.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0000002; Biological process: mitochondrial genome maintenance.

Cross-references [?]

Pfam PF05970; PIF1; 1-2;

Features [?]

From: PIF1_YEAST (P07271)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     258     265       ATP     G-x-A-G-T-G-K-S  
DNA_BIND     727     746             [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F  

Additional information [?]

Size range 593-1113 amino acids
Related rules MF_03177 (RRM3 supersedes the current rule)
Fusion None