HAMAP rule MF_03176
General rule information
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Accession | MF_03176 |
Dates | 31-JUL-2013 (Created) 19-NOV-2022 (Last updated, Version 10) |
Name | PIF1 |
Scope | Eukaryota |
Templates | P07271 (PIF1_YEAST); Q9H611 (PIF1_HUMAN); Q9UUA2 (PIF1_SCHPO): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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case <OC:Saccharomycetales>
Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing. |
else case <OC:Mammalia>
Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. |
else
Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. |
end case
Catalytic activity | RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
EC 3.6.4.12 |
Cofactor | Mg(2+) |
case <OC:Saccharomycetales> or <OC:Mammalia>
Subunit | Monomer. Interacts with telomerase. |
else
Subunit | Monomer. |
end case
Subcellular location | Nucleus. Mitochondrion. |
Similarity | Belongs to the helicase family. PIF1 subfamily. |
Keywords
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ATP-binding
DNA damage
DNA recombination
DNA repair
DNA-binding
Helicase
Hydrolase
Mitochondrion
Nucleotide-binding
Nucleus
DNA damage
DNA recombination
DNA repair
DNA-binding
Helicase
Hydrolase
Mitochondrion
Nucleotide-binding
Nucleus
Gene Ontology
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case <OC:Saccharomycetales> or <OC:Mammalia>
GO:0051880; Molecular function: G-quadruplex DNA binding.
GO:0010521; Molecular function: telomerase inhibitor activity.
GO:0032211; Biological process: negative regulation of telomere maintenance via telomerase.
GO:0010521; Molecular function: telomerase inhibitor activity.
GO:0032211; Biological process: negative regulation of telomere maintenance via telomerase.
end case
GO:0005739; Cellular component: mitochondrion.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0043139; Molecular function: 5'-3' DNA helicase activity.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0000002; Biological process: mitochondrial genome maintenance.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0043139; Molecular function: 5'-3' DNA helicase activity.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0000002; Biological process: mitochondrial genome maintenance.
Cross-references
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Pfam | PF05970; PIF1; 1-2; |
Features
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From: PIF1_YEAST (P07271) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 258 | 265 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-x-A-G-T-G-K-S | ||||||||
DNA_BIND | 727 | 746 | [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F |
Additional information
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Size range | 593-1113 amino acids |
Related rules | MF_03177 (RRM3 supersedes the current rule) |
Fusion | None |