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HAMAP rule MF_03222

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General rule information [?]

Accession MF_03222
Dates 20-OCT-2016 (Created)
1-JUN-2023 (Last updated, Version 8)
Name Succ_CoA_alpha_euk
Scope(s) Eukaryota
Template(s) O19069 (SUCA_PIG); Q9YGD2 (SUCA_COLLI); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01988 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SUCA
case <OC:Metazoa> or <OC:Amoebozoa>
Protein name RecName: Full=Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial;
                 EC=6.2.1.4;
                 EC=6.2.1.5;
AltName: Full=Succinyl-CoA synthetase subunit alpha;
                 Short=SCS-alpha;
else
Protein name RecName: Full=Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial;
                 EC=6.2.1.5;
AltName: Full=Succinyl-CoA synthetase subunit alpha;
                 Short=SCS-alpha;
end case
case <OC:Vertebrata>
Gene name Name=SUCLG1;
end case

Comments [?]

case <OC:Metazoa> or <OC:Amoebozoa>
FUNCTIONSuccinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.
CATALYTIC ACTIVITY Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4;
else
FUNCTIONSuccinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.
end case
CATALYTIC ACTIVITY Reaction=ATP + CoA + succinate = ADP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:17661, ChEBI:CHEBI:30031, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:456216; EC=6.2.1.5;
PATHWAYCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
case <OC:Vertebrata>
SUBUNITHeterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP- specific beta subunit @gn(SUCLA2), forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit @gn(SUCLG2) forms a GDP-forming succinyl-CoA synthetase (G-SCS).
else case <OC:Metazoa> or <OC:Amoebozoa>
SUBUNITHeterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP- specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A- SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).
else
SUBUNITHeterodimer of an alpha and a beta subunit.
end case
SUBCELLULAR LOCATIONMitochondrion.
SIMILARITYBelongs to the succinate/malate CoA ligase alpha subunit family.
case not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

Ligase
Mitochondrion
Nucleotide-binding
Tricarboxylic acid cycle

Gene Ontology [?]

GO:0004775; Molecular function:succinate-CoA ligase (ADP-forming) activity
GO:0006099; Biological process:tricarboxylic acid cycle

Cross-references [?]

PROSITE PS01216; SUCCINYL_COA_LIG_1; 1;
PROSITE PS00399; SUCCINYL_COA_LIG_2; 1;
Pfam PF02629; CoA_binding; 1;
Pfam PF00549; Ligase_CoA; 1;
Pfam PF13607; Succ_CoA_lig; 1;
PRINTS PR01798; SCOASYNTHASE; 1;
NCBIfam TIGR01019; sucCoAalpha; 1;
PIRSF PIRSF001553; SucCS_alpha; 1;
General TransitM; -; 0-1;

Features [?]

From: SUCA_PIG (O19069)
Key From To Description Tag Condition FTGroup
BINDING 64 67 /ligand="CoA"
/ligand_id="ChEBI:CHEBI:57287"		 T-G-x(2)
BINDING 143 145 /ligand="CoA"
/ligand_id="ChEBI:CHEBI:57287"		 [IVL]-T-[ED]
ACT_SITE 299 299 /note="Tele-phosphohistidine intermediate" H
BINDING 90 90 /ligand="CoA"
/ligand_id="ChEBI:CHEBI:57287"		 K
BINDING 207 207 /ligand="substrate"
/ligand_note="ligand shared with subunit beta"		 Y

Additional information [?]

Size range 299-347 amino acids
Related rules None
Fusion Nter: None Cter: None



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