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HAMAP rule MF_04052

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General rule information [?]

Accession MF_04052
Dates 9-MAR-2017 (Created)
25-FEB-2021 (Last updated, Version 4)
Name ADV_CAPSP
Scope
Viruses; Adenoviridae
Template P12538 (CAPSP_ADE05)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CAPSP
Protein name
RecName: Full=Penton protein;
Short=CP-P;
AltName: Full=Penton base protein;
AltName: Full=Protein III;
Gene name
L2

Comments [?]

case <FTGroup:1>
Function Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.
Subunit Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2. Interacts with the fiber protein (via N-terminal tail region). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons.
Subcellular location Virion. Host nucleus. Note=Located at each vertex of the virion. Present in 60 copies per virion.
Induction Expressed in the late phase of the viral replicative cycle.
Domain The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.
Miscellaneous All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Similarity Belongs to the adenoviridae penton family.
else
Function Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein, and in endocytosis of virions. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.
Subunit Interacts with the fiber protein (via N-terminal tail region). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons.
Subcellular location Virion. Host nucleus. Note=Located at each vertex of the virion.
Induction Expressed in the late phase of the viral replicative cycle.
Miscellaneous All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Similarity Belongs to the adenoviridae penton family.
end case

Keywords [?]

case <FTGroup:1>
Clathrin-mediated endocytosis of virus by host
end case
Host-virus interaction
Capsid protein
Host nucleus
Viral penetration into host cytoplasm
Late protein
T=25 icosahedral capsid protein
Viral attachment to host cell
Virion
Virus endocytosis by host
Virus entry into host cell

Gene Ontology [?]

case <FTGroup:1>
GO:0075512; Biological process: clathrin-dependent endocytosis of virus by host cell.
end case
GO:0042025; Cellular component: host cell nucleus.
GO:0039623; Cellular component: T=25 icosahedral viral capsid.
GO:0019028; Cellular component: viral capsid.
GO:0005198; Molecular function: structural molecule activity.
GO:0019062; Biological process: virion attachment to host cell.

Cross-references [?]

Pfam PF01686; Adeno_Penton_B; 1;

Features [?]

From: CAPSP_ADE05 (P12538)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     340     342       Cell attachment site     R-G-D   1

Additional information [?]

Size range 450-571 amino acids
Related rules None
Fusion None


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