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HAMAP rule MF_04101

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General rule information [?]

Accession MF_04101
Dates 28-JUN-2016 (Created)
19-NOV-2022 (Last updated, Version 8)
Name DPOL_T7
Scope
Viruses; Caudoviricetes
Template P00581 (DPOL_BPT7)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DPOL
Protein name
RecName: Full=DNA-directed DNA polymerase;
EC 2.7.7.7;
EC 3.1.11.-;

Comments [?]

Function Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction.
Catalytic activity RHEA:22508: a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
EC 2.7.7.7
Cofactor Mg(2+)
Similarity Belongs to the DNA polymerase type-A family.

Keywords [?]


Gene Ontology [?]

GO:0008408; Molecular function: 3'-5' exonuclease activity.
GO:0003677; Molecular function: DNA binding.
GO:0003887; Molecular function: DNA-directed DNA polymerase activity.
GO:0000166; Molecular function: nucleotide binding.

Cross-references [?]

PROSITE PS00447; DNA_POLYMERASE_A; 1;
Pfam PF00476; DNA_pol_A; 1;
PF01612; DNA_pol_A_exo1; 1;

Features [?]

From: DPOL_BPT7 (P00581)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     1     187       3'-5'exonuclease        
REGION     202     Cter       Polymerase        
BINDING     5     5       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic; for 3'-5' exonuclease activity     D  
BINDING     5     5       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity     D  
BINDING     7     7       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity     E  
BINDING     174     174       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity     D  
BINDING     475     475       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity     D  
BINDING     475     475       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity     D  
BINDING     476     476       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity     A  
BINDING     654     654       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity     D  
BINDING     654     654       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity     D  
BINDING     506     506       /ligand="substrate     H  
BINDING     518     518       /ligand="substrate     R  
BINDING     522     522       /ligand="substrate     K  
BINDING     526     526       /ligand="substrate     Y  

Additional information [?]

Size range 614-904 amino acids
Related rules None
Fusion None