HAMAP rule MF_04101
General rule information
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| Accession | MF_04101 |
| Dates | 28-JUN-2016 (Created)
19-NOV-2022 (Last updated, Version 8) |
| Name | DPOL_T7 |
| Scope(s) |
Viruses Caudoviricetes |
| Template(s) | P00581 (DPOL_BPT7); [ Recover all ] |
| Triggered by |
HAMAP; MF_04101 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Comments
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| FUNCTION | Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. |
| CATALYTIC ACTIVITY | Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.7; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SIMILARITY | Belongs to the DNA polymerase type-A family. |
Keywords
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| DNA replication |
| DNA-binding |
| DNA-directed DNA polymerase |
| Exonuclease |
| Hydrolase |
| Magnesium |
| Metal-binding |
| Multifunctional enzyme |
| Nuclease |
| Nucleotidyltransferase |
| Transferase |
| Viral DNA replication |
Gene Ontology
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| GO:0008408; Molecular function:3'-5' exonuclease activity |
| GO:0003677; Molecular function:DNA binding |
| GO:0003887; Molecular function:DNA-directed DNA polymerase activity |
| GO:0000166; Molecular function:nucleotide binding |
Cross-references
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Features
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| From: DPOL_BPT7 (P00581) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 1 | 187 | /note="3'-5'exonuclease" | |||||||||
| REGION | 202 | Cter | /note="Polymerase" | |||||||||
| BINDING | 5 | 5 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic; for 3'-5' exonuclease activity" |
D | ||||||||
| BINDING | 5 | 5 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity" |
D | ||||||||
| BINDING | 7 | 7 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity" |
E | ||||||||
| BINDING | 174 | 174 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity" |
D | ||||||||
| BINDING | 475 | 475 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity" |
D | ||||||||
| BINDING | 475 | 475 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity" |
D | ||||||||
| BINDING | 476 | 476 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity" |
A | ||||||||
| BINDING | 654 | 654 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity" |
D | ||||||||
| BINDING | 654 | 654 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity" |
D | ||||||||
| BINDING | 506 | 506 | /ligand="substrate" | H | ||||||||
| BINDING | 518 | 518 | /ligand="substrate" | R | ||||||||
| BINDING | 522 | 522 | /ligand="substrate" | K | ||||||||
| BINDING | 526 | 526 | /ligand="substrate" | Y | ||||||||
Additional information
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| Size range | 614-904 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |