HAMAP rule MF_04101

General rule information [?]

Accession	MF_04101
Dates	28-JUN-2016 (Created) 19-NOV-2022 (Last updated, Version 8)

Name

DPOL_T7

Scope

Viruses; Caudoviricetes

Template

P00581 (DPOL_BPT7)

Triggered by

HAMAP; MF_04101 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DPOL

Protein name

RecName:		Full=DNA-directed DNA polymerase;
		EC 2.7.7.7;
		EC 3.1.11.-;

Comments [?]

Function	Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction.
Catalytic activity	RHEA:22508: a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) EC 2.7.7.7
Cofactor	Mg(2+)
Similarity	Belongs to the DNA polymerase type-A family.

Keywords [?]

DNA replication
DNA-binding
DNA-directed DNA polymerase
Exonuclease
Hydrolase
Magnesium
Metal-binding
Multifunctional enzyme
Nuclease
Nucleotidyltransferase
Transferase
Viral DNA replication

Gene Ontology [?]

GO:0008408; Molecular function: 3'-5' exonuclease activity.
GO:0003677; Molecular function: DNA binding.
GO:0003887; Molecular function: DNA-directed DNA polymerase activity.
GO:0000166; Molecular function: nucleotide binding.

Cross-references [?]

PROSITE	PS00447; DNA_POLYMERASE_A; 1;
Pfam	PF00476; DNA_pol_A; 1;
	PF01612; DNA_pol_A_exo1; 1;

Features [?]

From: DPOL_BPT7 (P00581)

Key From To Description Tag Condition FTGroup

REGION 1 187 3'-5'exonuclease

REGION 202 Cter Polymerase

BINDING 5 5 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic; for 3'-5' exonuclease activity D

BINDING 5 5 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity D

BINDING 7 7 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity E

BINDING 174 174 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for 3'-5' exonuclease activity D

BINDING 475 475 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity D

BINDING 475 475 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity D

BINDING 476 476 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity A

BINDING 654 654 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="3" /ligand_note="catalytic; for polymerase activity D

BINDING 654 654 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="4" /ligand_note="catalytic; for polymerase activity D

BINDING 506 506 /ligand="substrate H

BINDING 518 518 /ligand="substrate R

BINDING 522 522 /ligand="substrate K

BINDING 526 526 /ligand="substrate Y

Additional information [?]

Size range	614-904 amino acids
Related rules	None
Fusion	None

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