HAMAP rule MF_04168
General rule information
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Accession | MF_04168 |
Dates | 16-JUL-2021 (Created) 1-DEC-2022 (Last updated, Version 5) |
Name | HOLIN_D29 |
Scope | Viruses; Caudoviricetes |
Template | O64204 (HOLIN_BPMD2) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Comments
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Function | Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Participates with the endolysin protein in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell. |
Subunit | Homomultimer. Self-associates to form a pore. |
case <AnyFeature:Transmembrane>=2> and <Feature:Coiled_coil>
Subcellular location | Host cell inner membrane; Multi-pass membrane protein. |
Domain | The first transmembrane region undergoes a helix to beta-hairpin conformational change, which is responsible for its self-association and pore formation in the host membrane. The coiled coil region is required for host cell lysis and for cytotoxic activity. The C-terminus determines the size of the hole. |
end case
case <AnyFeature:Transmembrane>=2> and not <Feature:Coiled_coil>
Subcellular location | Host cell inner membrane; Multi-pass membrane protein. |
Domain | The first transmembrane region undergoes a helix to beta-hairpin conformational change, which is responsible for its self-association and pore formation in the host membrane. The C-terminus determines the size of the hole. |
end case
case <AnyFeature:Transmembrane<2> and <Feature:Coiled_coil>
Subcellular location | Host cell inner membrane. |
Domain | The coiled coil region is required for host cell lysis and for cytotoxic activity. The C-terminus determines the size of the hole. |
end case
case <AnyFeature:Transmembrane<2> and not <Feature:Coiled_coil>
Subcellular location | Host cell inner membrane. |
Domain | The C-terminus determines the size of the hole. |
end case
Similarity | Belongs to the D29 holin family. |
Keywords
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case <Feature:coiled>
end case
Cytolysis
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Membrane
Transmembrane
Transmembrane helix
Viral release from host cell
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Membrane
Transmembrane
Transmembrane helix
Viral release from host cell
Gene Ontology
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GO:0140911; Molecular function: pore-forming activity.
GO:0016020; Cellular component: membrane.
GO:0020002; Cellular component: host cell plasma membrane.
GO:0016020; Cellular component: membrane.
GO:0020002; Cellular component: host cell plasma membrane.
Cross-references
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Pfam | PF16081; Phage_holin_7_1; 1; |
Computed features
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General | Transmembrane; -; 2; |
Coiled_coil; -; 0-unlimited; |
Additional information
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Size range | 120-170 amino acids |
Related rules | None |
Fusion | None |