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Annotation rule MF_04168
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General rule information [?]

Accession MF_04168
Dates 16-JUL-2021 (Created)
29-SEP-2022 (Last updated, Version 4)
Name HOLIN_D29
Scope
Viruses; Caudoviricetes
Template O64204 (HOLIN_BPMD2)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HOLIN
Protein name
RecName: Full=Holin;

Comments [?]

Function Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Participates with the endolysin protein in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.
Subunit Homomultimer. Self-associates to form a pore.
case <AnyFeature:Transmembrane>=2> and <Feature:Coiled_coil>
Subcellular location Host cell inner membrane; Multi-pass membrane protein.
Domain The first transmembrane region undergoes a helix to beta-hairpin conformational change, which is responsible for its self-association and pore formation in the host membrane. The coiled coil region is required for host cell lysis and for cytotoxic activity. The C-terminus determines the size of the hole.
end case
case <AnyFeature:Transmembrane>=2> and not <Feature:Coiled_coil>
Subcellular location Host cell inner membrane; Multi-pass membrane protein.
Domain The first transmembrane region undergoes a helix to beta-hairpin conformational change, which is responsible for its self-association and pore formation in the host membrane. The C-terminus determines the size of the hole.
end case
case <AnyFeature:Transmembrane<2> and <Feature:Coiled_coil>
Subcellular location Host cell inner membrane.
Domain The coiled coil region is required for host cell lysis and for cytotoxic activity. The C-terminus determines the size of the hole.
end case
case <AnyFeature:Transmembrane<2> and not <Feature:Coiled_coil>
Subcellular location Host cell inner membrane.
Domain The C-terminus determines the size of the hole.
end case
Similarity Belongs to the D29 holin family.

Keywords [?]

case <Feature:coiled>
Coiled coil
end case
Cytolysis
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Membrane
Transmembrane
Transmembrane helix
Viral release from host cell

Gene Ontology [?]

GO:0034291; Molecular function: canonical holin activity.
GO:0016020; Cellular component: membrane.
GO:0020002; Cellular component: host cell plasma membrane.

Cross-references [?]

Pfam PF16081; Phage_holin_7_1; 1;

Computed features [?]

General Transmembrane; -; 2;
Coiled_coil; -; 0-unlimited;

Additional information [?]

Size range 120-170 amino acids
Related rules None
Fusion None


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