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HAMAP rule MF_00379

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General rule information [?]

Accession MF_00379
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36)
Name GTPase_MnmE
Scope(s) Bacteria
Plastid
Template(s) P25522 (MNME_ECOLI); Q9WYA4 (MNME_THEMA); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OG:Chloroplast>
HAMAP; MF_00379 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MNME
case <OC:Bacteria>
Protein name RecName: Full=tRNA modification GTPase MnmE;
                 EC=3.6.-.-;
Gene name Name=mnmE; Synonyms=trmE;
else case <OG:Chloroplast>
Protein name RecName: Full=Probable tRNA modification GTPase MnmE;
                 EC=3.6.-.-;
Gene name Name=mnmE; Synonyms=thdF;
end case

Comments [?]

FUNCTIONExhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34.
COFACTOR Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Binds 1 potassium ion per subunit.;
case <OC:Bacteria>
SUBUNITHomodimer. Heterotetramer of two MnmE and two MnmG subunits.
SUBCELLULAR LOCATIONCytoplasm.
else case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
SIMILARITYBelongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.

Keywords [?]


Gene Ontology [?]

GO:0005525; Molecular function:GTP binding
GO:0003924; Molecular function:GTPase activity
GO:0006400; Biological process:tRNA modification
case <OCellular component:Bacteria>
GO:0005737; Cellular component:cytoplasm
else case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam PF01926; MMR_HSR1; 1;
Pfam PF10396; TrmE_N; 1;
PRINTS PR00326; GTP1OBG; 1;
NCBIfam TIGR00450; MnmE_trmE_thdF; 1;
NCBIfam TIGR00231; Small_GTP; 1;
PROSITE PS51709; G_TRME; 1;

Features [?]

From: MNME_ECOLI (P25522)
Key From To Description Tag Condition FTGroup
BINDING 226 231 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
N-[VSAT]-G-K-S-[TSQR]
BINDING 245 251 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
[TSMAD]-x(3)-G-[TSR]-T
BINDING 270 273 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
D-[TS]-A-G
BINDING 335 338 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
N-K-A-D
BINDING 358 360 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
S-A-R
BINDING 226 226 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"
N 1
BINDING 230 230 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
S
BINDING 245 245 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"
[TS] 1
BINDING 247 247 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"
[IVL] 1
BINDING 250 250 /ligand="K(+)"
/ligand_id="ChEBI:CHEBI:29103"
[TS] 1
BINDING 251 251 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
T
BINDING 23 23 /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
/ligand_id="ChEBI:CHEBI:57457"
[RK] 2
BINDING 80 80 /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
/ligand_id="ChEBI:CHEBI:57457"
E 2
BINDING 120 120 /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
/ligand_id="ChEBI:CHEBI:57457"
[RKVM] 2
BINDING 454 454 /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
/ligand_id="ChEBI:CHEBI:57457"
K 2

Additional information [?]

Size range 419-550 amino acids
Related rules None
Fusion Nter: None Cter: None



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