HAMAP rule MF_01129

General rule information [?]

Accession	MF_01129
Dates	22-JUL-2003 (Created) 28-JUL-2023 (Last updated, Version 47)
Name	PPase_energized_pump
Scope(s)	Bacteria Archaea
Template(s)	Q9S5X0 (HPPA_THEMA); Q3AFC6 (HPPA_CARHZ); Q8ZWI8 (HPPA_PYRAE); Q8PYZ8 (HPPA1_METMA); [ Recover all ]
Triggered by	HAMAP; MF_01129 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	HPPA
case <FTTag:Kdep>
Protein name	RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump; EC=7.2.3.1; AltName: Full=Membrane-bound sodium-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=Na(+)-PPase;
else case <FTTag:Kindep>
Protein name	RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump; EC=7.1.3.1; AltName: Full=Membrane-bound proton-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=H(+)-PPase;
else
Protein name	RecName: Full=Pyrophosphate-energized proton pump; EC=7.1.3.1; AltName: Full=Membrane-bound proton-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=H(+)-PPase;
end case
Gene name	Name=hppA;

Comments [?]

case <FTTag:Kdep>
FUNCTION	Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane.
CATALYTIC ACTIVITY	Reaction=diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2 phosphate; Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.2.3.1;
else
FUNCTION	Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.
CATALYTIC ACTIVITY	Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate; Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
end case
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
case <FTTag:Kdep>
ACTIVITY REGULATION	Requires K(+) for maximal activity.
end case
SUBUNIT	Homodimer.
case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATION	Cell membrane; Multi-pass membrane protein.
else case <Property:Membrane=2>
SUBCELLULAR LOCATION	Cell inner membrane; Multi-pass membrane protein.
end case
case <FTTag:Kdep>
SIMILARITY	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily.
else case <FTTag:Kindep>
SIMILARITY	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily.
else
SIMILARITY	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family.
end case

Keywords [?]

case defined <Property:Membrane> and <Property:Membrane=2>
Cell inner membrane
end case
Cell membrane
Ion transport
Magnesium
Membrane
case <FTTag:Kdep>
Potassium
Sodium
Sodium transport
else
Hydrogen ion transport
end case
<a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a>
Transport
Transmembrane
Transmembrane helix

Gene Ontology [?]

GO:0009678; Molecular function:diphosphate hydrolysis-driven proton transmembrane transporter activity
GO:0004427; Molecular function:inorganic diphosphate phosphatase activity
GO:0000287; Molecular function:magnesium ion binding
case <FTTag:Kdep>
GO:0030955; Molecular function:potassium ion binding
GO:0006814; Biological process:sodium ion transport
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:1902600; Biological process:proton transmembrane transport
end case
GO:0005886; Cellular component:plasma membrane

Cross-references [?]

Features [?]

From: HPPA_THEMA (Q9S5X0)

Key

From

Description

Tag

Condition

FTGroup

SITE

495

/note="Determinant of potassium dependence"

Kdep

From: HPPA_PYRAE (Q8ZWI8)

Key

From

Description

Tag

Condition

FTGroup

SITE

476

/note="Determinant of potassium independence"

Kindep

Additional information [?]

Size range	655-825 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	There are two classes of pyrophosphate-energized pumps, differing in K(+) sensitivity (PubMed:17454298). Three members of the K(+)-stimulated subfamily have been characterized as sodium pumps (PubMed:17605473). Further characterization is needed to distinguish Na(+) transporters from H(+) transporters.