HAMAP rule MF_01349
General rule information
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Accession | MF_01349 |
Dates | 29-MAY-2006 (Created)
1-JUN-2023 (Last updated, Version 22) |
Name | PanCY |
Scope(s) |
Bacteria Cyanobacteriota |
Template(s) | P9WIL5 (PANC_MYCTU); P0A6I0 (KCY_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01349 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | PANCY |
Protein name | RecName: Full=Bifunctional pantoate ligase/cytidylate kinase; Includes: RecName: Full=Pantothenate synthetase; Short=PS; EC=6.3.2.1; AltName: Full=Pantoate--beta-alanine ligase; AltName: Full=Pantoate-activating enzyme; Includes: RecName: Full=Cytidylate kinase; Short=CK; EC=2.7.4.25; AltName: Full=Cytidine monophosphate kinase; Short=CMP kinase; |
Gene name | Name=panC/cmk; |
Comments
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FUNCTION | Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate. |
FUNCTION | Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively. |
CATALYTIC ACTIVITY | Reaction=(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10912, ChEBI:CHEBI:15378, ChEBI:CHEBI:15980, ChEBI:CHEBI:29032, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57966, ChEBI:CHEBI:456215; EC=6.3.2.1; |
CATALYTIC ACTIVITY | Reaction=ATP + CMP = ADP + CDP; Xref=Rhea:RHEA:11600, ChEBI:CHEBI:30616, ChEBI:CHEBI:58069, ChEBI:CHEBI:60377, ChEBI:CHEBI:456216; EC=2.7.4.25; |
CATALYTIC ACTIVITY | Reaction=ATP + dCMP = ADP + dCDP; Xref=Rhea:RHEA:25094, ChEBI:CHEBI:30616, ChEBI:CHEBI:57566, ChEBI:CHEBI:58593, ChEBI:CHEBI:456216; EC=2.7.4.25; |
PATHWAY | Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)- pantothenate from (R)-pantoate and beta-alanine: step 1/1. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | In the N-terminal section; belongs to the pantothenate synthetase family. |
SIMILARITY | In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily. |
Keywords
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Cytoplasm |
ATP-binding |
Kinase |
Ligase |
Multifunctional enzyme |
Nucleotide-binding |
Pantothenate biosynthesis |
Transferase |
Gene Ontology
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GO:0004592; Molecular function:pantoate-beta-alanine ligase activity |
GO:0005524; Molecular function:ATP binding |
GO:0004127; Molecular function:cytidylate kinase activity |
GO:0006220; Biological process:pyrimidine nucleotide metabolic process |
GO:0015940; Biological process:pantothenate biosynthetic process |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF02224; Cytidylate_kin; 1; |
Pfam | PF02569; Pantoate_ligase; 1; |
NCBIfam | TIGR00017; Cmk; 1; |
NCBIfam | TIGR00018; PanC; 1; |
NCBIfam | TIGR00125; Cyt_tran_rel; 1; |
Features
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From: PANCY_SYNY3 (Q55074) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 30 | 37 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
M-x(3)-H-x(2)-H | ||||||||
BINDING | 150 | 153 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x(2)-D | ||||||||
BINDING | 187 | 190 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
x(3)-R | ||||||||
REGION | Nter | 283 | /note="Pantoate--beta-alanine ligase" | |||||||||
REGION | 284 | Cter | /note="Cytidylate kinase" | |||||||||
ACT_SITE | 37 | 37 | /note="Proton donor" | H | ||||||||
BINDING | 61 | 61 | /ligand="beta-alanine" /ligand_id="ChEBI:CHEBI:57966" |
Q | ||||||||
BINDING | 61 | 61 | /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980" |
Q | ||||||||
BINDING | 156 | 156 | /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980" |
Q | ||||||||
BINDING | 179 | 179 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[VLIGA] |
Additional information
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Size range | 480-542 amino acids |
Related rules |
MF_00158 MF_00238 MF_00239 |
Fusion | Nter: None Cter: None |
Comments | This fusion protein seems only to exist in Cyanobacteriota; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity. |