HAMAP rule MF_01397
General rule information
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| Accession | MF_01397 |
| Dates | 29-JAN-2009 (Created)
1-JUN-2023 (Last updated, Version 12) |
| Name | NDH1_NuoCD_2 |
| Scope(s) |
Bacteria Aquificae Bacteroidia Deltaproteobacteria Campylobacteraceae |
| Template(s) | P33599 (NUOCD_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01397 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NUOCD |
| Protein name | RecName: Full=NADH-quinone oxidoreductase subunit C/D; EC=7.1.1.-; AltName: Full=NADH dehydrogenase I subunit C/D; AltName: Full=NDH-1 subunit C/D; AltName: Full=NUO3/NUO4; |
| Gene name | Name=nuoC; |
Comments
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| FUNCTION | NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. |
| CATALYTIC ACTIVITY | Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; |
| SUBUNIT | NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex. |
| SUBCELLULAR LOCATION | Cell inner membrane; Peripheral membrane protein. |
| SIMILARITY | In the N-terminal section; belongs to the complex I 30 kDa subunit family. |
| SIMILARITY | In the C-terminal section; belongs to the complex I 49 kDa subunit family. |
Keywords
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| Cell inner membrane | |
| Cell membrane | |
| Cytoplasm | |
| Membrane | |
| Multifunctional enzyme | |
| NAD | |
| Quinone | |
| <a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a> | |
| Transport | |
| Ubiquinone | |
Gene Ontology
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| GO:0016655; Molecular function:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor |
| GO:0050136; Molecular function:NADH dehydrogenase (quinone) activity |
| GO:0005886; Cellular component:plasma membrane |
Cross-references
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| PROSITE | PS00542; COMPLEX1_30K; 1; |
| PROSITE | PS00535; COMPLEX1_49K; 1; |
| Pfam | PF00329; Complex1_30kDa; 1; |
| Pfam | PF00346; Complex1_49kDa; 1; |
| NCBIfam | TIGR01961; NuoC_fam; 1; |
| NCBIfam | TIGR01962; NuoD; 1; |
Features
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| From: NUOCD_BDEBA (Q6MIR5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 157 | /note="NADH dehydrogenase I subunit C" | |||||||||
| REGION | 175 | Cter | /note="NADH dehydrogenase I subunit D" | |||||||||
Additional information
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| Size range | 519-593 amino acids |
| Related rules |
MF_01357 MF_01358 MF_01359 |
| Fusion | Nter: None Cter: None |
| Comments | This family is specific for the nuoC-nuoD fusion found in Aquificae, Bacteroidetes, Delta-and Epsilonproteobacteria. The nuoCD fusion protein found in the Alpha-, Beta- and Gammaproteobacteria is different enough that it seems to be another subfamily (MF_01359). The individual families are MF_01357 (nuoC) and MF_01358 (nuoD). In many bacteria there are both the individual and fused versions. 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. |