HAMAP rule MF_01438
General rule information
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| Accession | MF_01438 |
| Dates | 7-JUN-2006 (Created)
13-JAN-2023 (Last updated, Version 20) |
| Name | DisA |
| Scope(s) |
Bacteria Actinomycetota Bacillaceae Clostridia Fusobacterium Thermotoga |
| Template(s) | P37573 (DISA_BACSU); Q9WY43 (DISA_THEMA); P9WNW5 (DISA_MYCTU); A0R564 (DISA_MYCS2); Q9X8L6 (DISA_STRCO); A0R8F5 (DISA_BACAH); Q6HPT4 (DISA_BACHK); [ Recover all ] |
| Triggered by |
HAMAP; MF_01438 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DISA |
| Protein name | RecName: Full=DNA integrity scanning protein DisA; AltName: Full=Diadenylate cyclase; EC=2.7.7.85; AltName: Full=Cyclic di-AMP synthase; Short=c-di-AMP synthase; |
| Gene name | Name=disA; |
Comments
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| case <OC:Bacillaceae> or <OC:Clostridia> or <OC:Frankia> or <OC:Streptomyces> | |
| FUNCTION | Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. DisA forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation. |
| FUNCTION | Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome, operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis. |
| else | |
| FUNCTION | Participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions. |
| FUNCTION | Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP likely acts as a signaling molecule that may couple DNA integrity with a cellular process. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Homooctamer. |
| SIMILARITY | Belongs to the DisA family. |
Keywords
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| ATP-binding |
| DNA damage |
| DNA repair |
| DNA-binding |
| Nucleotide-binding |
| Nucleotidyltransferase |
| Magnesium |
| Transferase |
Gene Ontology
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| GO:0003677; Molecular function:DNA binding |
| GO:0005524; Molecular function:ATP binding |
| GO:0016779; Molecular function:nucleotidyltransferase activity |
| GO:0006281; Biological process:DNA repair |
| GO:0019932; Biological process:second-messenger-mediated signaling |
Cross-references
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| PROSITE | PS51794; DAC; 1; |
| Pfam | PF10635; DisA-linker; 1; |
| Pfam | PF02457; DisA_N; 1; |
| Pfam | PF00633; HHH; 1; |
Features
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| From: DISA_THEMA (Q9WY43) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 107 | 111 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
x-R-x-x-[TS] | ||||||||
| BINDING | 76 | 76 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 94 | 94 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[LV] | ||||||||
Additional information
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| Size range | 344-374 amino acids |
| Related rules |
MF_00838 MF_00840 MF_01499 |
| Fusion | Nter: None Cter: None |