HAMAP rule MF_02106
General rule information
[?]
| Accession | MF_02106 |
| Dates | 7-DEC-2009 (Created)
27-JUL-2023 (Last updated, Version 12) |
| Name | Pup |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | P9WHN5 (PUP_MYCTU); A0QZ48 (PUP_MYCS2); Q8NQE0 (PUP_CORGL); [ Recover all ] |
| Triggered by |
HAMAP; MF_02106 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | PUP |
| Protein name | RecName: Full=Prokaryotic ubiquitin-like protein Pup; AltName: Full=Bacterial ubiquitin-like modifier; |
| Gene name | Name=pup; |
Comments
[?]
| FUNCTION | Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. |
| PATHWAY | Protein degradation; proteasomal Pup-dependent pathway. |
| SUBUNIT | Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring. |
| DOMAIN | The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome. |
| case <FTTag:deamidation> | |
| PTM | Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process. |
| end case | |
| SIMILARITY | Belongs to the prokaryotic ubiquitin-like protein family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0031386; Molecular function:protein tag activity |
| GO:0070628; Molecular function:proteasome binding |
| GO:0010498; Biological process:proteasomal protein catabolic process |
| GO:0019941; Biological process:modification-dependent protein catabolic process |
| GO:0070490; Biological process:protein pupylation |
Cross-references
[?]
| Pfam | PF05639; Pup; 1; |
| General | Coiled_coil; -; 0-unlimited; |
Features
[?]
| From: PUP_MYCTU (P9WHN5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 21 | 58 | /note="ARC ATPase binding" | |||||||||
| MOD_RES | 64 | 64 | /note="Deamidated glutamine" | deamidation | Q | |||||||
| case <FT:2> | ||||||||||||
| CROSSLNK | 64 | 64 | /note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins)" | [Q] | ||||||||
| else | ||||||||||||
| CROSSLNK | 64 | 64 | /note="Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-? in acceptor proteins)" | [E] | ||||||||
| end case | ||||||||||||
Additional information
[?]
| Size range | 62-72 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process. |